DEVELOPMENT OF ROSSET-TYPE STERILE WATER SAMPLER FROM DIFFERENT DEPTHS FOR MICROBIOLOGICAL RESEARCH

研制了一种用于微生物研究的新型无菌取水器。该采样器的特点是：使用深度达6000米，可从15个不同的层取样，最大容量为1000毫升。它由带超声波发生器和换能器的船上设备和带夹紧单元、切割单元、驱动单元、泵单元、控制单元、发电单元和全密闭水箱的水下设备组成。车载设备发出的超声波将启动取水器，切断进水口的管子，然后泵机组将全密性水箱内的水吸出，在负压下，环境水样通过硅胶管进入水箱内的样品袋中。控制单元将对取样器的时间进行操作。这一步骤将在15个不同的层面上继续进行，当然，内部的所有系统都已经预先消毒。我们已经成功地在KH-00-1号邮轮和KT-00-9号邮轮期间采集了6,000米以下的水样。

A very new type of sterile water sampler for microbiological research has been developed. Specificaton for this sampler is as follows ; depth of the use is up to 6,000 m, capable of sampling from 15 different layers, and maximum volume of 1,000 ml. It is composed of onboard facility with ultrasonic generator and transducer, and under water facility with cramp unit, cutter unit, drive unit, pump unit, control unit, generator unit and a all-tight water tank. Ultrasonic wave sent from onboard facility will activate the water sampler and cut the tubing at the water entrance, and then pump unit will suck out water inside the all-tight water tank which will induce ambient water sarnple to introduce into sample bag inside the tank through silicone tubing by negative pressure. Control unit will operate the time for the sampler. This step will be continued at 15 different layers, All the system inside has been sterilized beforehand of course. We have successfully sampled water from surface to 6,000 m during KH-00-1 cruise by R/V Hakuho-maru and KT-00-9 cruise by R/V Tansei-maru.