CONSTRUCTION OF THE DEEP-SEA MICROBIAL IN SITU INCUBATION SYSTEM TO SEARCH FOR DIVERSIFIED DEEP-SEA BAROPHILIC MICROORGANISMS

深海微生物原位培养系统的建造具有以下特点：最大使用深度：6000米最长系泊时间：1年重量和尺寸：尽可能小和轻，使潜水器能够带到深海海底操作：电池和计时器水样：管泵系统(大和，日本)孵化器：8套无菌“血袋系统”(TERFLEX，日本)，每套150毫升；8套孵化器，预先添加小体积的不同基质，并将孵化器的所有管子连接到管泵系统。计时器被设置为在系泊到深海后的第二天开始运行。它是由定时器操作的耐压电池房内的电池迫使电机旋转，使切割器旋转并切断通往孵化器的所有进料管，并使管道泵送系统与孵化器相连，以获取…中的环境水1996年11月21日，深海微生物原位培养系统停泊在日本海沟5,000米处，位于北纬38ﾟ01‘，东经145ﾟ32’，在R/V白丸巡航期间，在不同深度安装了多套水流计。1997年9月2日，在设置了大约9个月后，R/V Tansei-Maru在巡航过程中成功地在船上找回了它。整个孵化系统是安全的。然而，不幸的是，在8套油管抽水系统中，只有一套工作正常，其他7套完全没有将深海水样带入培养袋中。在一个以Zobell 2216E为底物的培养袋中，观察到深海细菌的密集生长(1.1×10~(-8)个/ml)。在1atm的条件下进行实验培养，没有显示出任何生长。相反，它们在2℃的加压条件下(500atm)生长良好。这些菌株的分离和系统发育研究正在进行中。较少

The Deep-Sea Microbial in situ Incubation System was constructed with the following specificaton ;Maximum depth of use : 6,000 m Maximum duration of mooring : 1 year Weight and size : as small and light as possible so that submersible can bring to the deep-sea floor Operation : battery and timer Water sampling : tubing pump system (Yamato, Co., Japan) Incubators : 8 sets of sterile " Blood Bag System " (TERFLEX,Japan) with volume of 150 ml eachInto the 8 sets of incubators, small volume of the different substrates were added beforehand, and all the tubes from the incubators were connected to the tube pumping systems. The timer was set to start operation one day after the mooring to the deep-ocean. It was constructed that the battery inside the pressure tolerant battery house operated by a timer forces the motor to rotate which makes the cutter to rotate and cut off all the intake tubes to incubators and makes the tube pumping systems connected to the incubators to take ambient water in … More to the incubators.The Deep-Sea Microbial in situ Incubation System was moored at the site of the Japan Trench at a depth of 5,000 m on November 21,1996, at 38ﾟ01'N,145ﾟ32'E with several sets of water current meters at different depths during R/V Hakuho-maru cruise. On September 2,1997, it was successfully retrieved on board the vessel during cruise by R/V Tansei-maru after setting it for about 9 months. Whole set of incubation system was safe. Unfortunately, however, it was found that in the 8 sets of tubing pumping systems only one worked well and other 7 sets didn't take any deep-sea water sample at all into incubation bags. In one incubation bag in which ZoBell 2216 E was added as a substrate, dense growth of deep-sea bacteria (1.1x10^8 cells/ml) was observed. Experimental culture of them under 1 atm condition did not show any growth at all. On the contrary they showed good growth under pressurized condition (500 atm) at 2 C.Isolation of these bacterial strains and study for phylogeny of them are now undertaken. Less