HEALTH RISK ASSESSMENT OF ENDOCRINE DISRUPTERS BASED ON DEVELOPMENT, ARCHITECUTURE AND PHYSIOLOGICAL FUNCTION IN THE LUNG.

基于肺的发育、结构和生理功能，研究了内分泌干扰物(EDCs)专用健康评估的开发。结果：1.用己烯雌酚(DES)处理DAME所生的BALB/c雌性小鼠，6种不同品系的小鼠体重增加明显。与BALB/c对照组相比，经DES处理后10日龄DAME出生的女婴胸腺重量和脾中B细胞百分比明显增加。3.经DES处理后10日龄DAME出生的小鼠肺组织中来源于Clara细胞的细胞色素P2F2、P450还原酶、III型和IV型胶原mRNAs的表达均低于未处理组。4.经DES处理的6株DAME所产婴儿的心肺组织病理改变与相应对照组无显著差异。5.用体外培养系统…检测3-甲基胆蒽(3MC)对骨代谢的影响更多的艾米。3MC抑制成骨细胞和破骨细胞的分化和增殖。结果提示，3mc可能对骨代谢起关键作用。以小鼠胚胎为研究对象，研究了内皮细胞对胚胎成骨的影响。在EDCs处理的小鼠胚胎中，骨形态异常和骨化延迟。颈椎、胸椎和四肢出现延迟骨化。在四肢中，在掌骨和/或跖骨中观察到延迟骨化。有EDCs的前肢第二和/或第五掌骨的骨基质较对照前肢的骨基质薄。DES与骨异常发生率的量效关系尚不清楚。7.比较DAME治疗组和非DAME组大鼠的肺功能。至出生后9周，两组间差异无统计学意义。8.研究了不同来源的内分泌细胞对日本涡虫、小鼠细胞培养和小鼠胚胎细胞重构能力的影响。EDCs对非哺乳动物和哺乳动物的毒性作用之间的关系并不总是一致的。这些结果表明，在骨骼建模的基础上，EDCs处理的小鼠胚胎成熟过程中肺的发育和构筑被推迟。直到产后9周，没有证据表明肺功能有变化。胎儿骨骼形态和骨化程度可作为EDCs毒性健康评估的较好生物学指标。较少

Development of an appropriative health assessment for endocrine disrupters (EDCs) was studied based on development, architecture, and physiological functions of the lung.1. BALB/c female mice born to dame treated with diethyistilbestrol (DES) demonstrated significant increase of body weight gain with six different strains of mice.2. Female babies born to dame treated with DES showed increases of thymus weight and percentage of B-cells in spleen compared with those of the control group of BALB/c. 3. Expressions of CYP2F2 derived from Clara cells, P450 reductase, collagen III and IV mRNAs in the lung were reduced in 10-days-old babies born from dam treated with DES compared with non-treated mice. 4. Pathological changes in cardiopulmonary tissues of babies born to six strains of dame treated with DES were not significantly different from those of corresponding control groups. 5. The effects of 3- methylcholanthrene (3MC) on the metabolism of bone were examined using in vitro culture syst … More em. 3MC decreased differentiation and proliferation of osteoblasts and osteoclasts. Results suggested that 3MC might exert critical effects on bone metabolism.6. The effects of EDCs on formation of bone were studied in mouse fetuses. Abnormalities of bone morphology and delay in ossification were shown in mouse fetuses treated with EDCs. Delayed ossification was seen in the cervical and thoracic vertebrae and limbs. In the limbs, delayed ossification was observed in the metacarpals and/or metatarsals. Bone matrix in the second and/or fifth metacarpals in the forelimbs with EDCs was thinner than that in the forelimbs with control. Dose- response of DES relative to incidence of abnormalities of bone was not clear. 7. The pulmonary functions were compared in babies born to dame treated with DES and those babies born to non-treated dame in rats. There was no significant difference between the two groups up to 9 weeks after birth. 8. Effects of various EDCs were investigated with respect to abilities to restructure in Dugesia japonica, cell culture derived from mice, and fetal mice. The relationships between the toxic effects of EDCs in non mammals and mammals do not always coincide.These results suggested that the development and architecture of the lung during maturation of fetuses in mice treated with EDCs was delayed based on the modeling of bone. There is no evidence of changes in pulmonary functions up to 9 weeks post partum. Bone morphology and ossification in fetus may serve as better biological markers to health assessment in EDCs toxicity. Less