The expression of a Novel Stress Protein "150-kD a Oxygen Regulated Protein (ORP-150) "in Forensic Autopsy Cases

氧调节蛋白150-kDa（ORP-150）仅在缺氧条件下被诱导。应用免疫组织化学和形态计量学方法对婴儿猝死（SID）患者脑组织中ORP-150的表达进行了研究。18名婴儿的大脑皮层用于这项研究。将每个组织切片与抗ORP-150多克隆抗体孵育，并计数ORP-150阳性细胞的数量。聚类分析将18例患者分为3组（A-C组）。A组为6例婴儿猝死综合征（SIDS），ORP-150阳性细胞平均值为66.75 ± 3.44; B组为6例肺炎、支气管炎等严重呼吸道感染性疾病（包括败血症），ORP-150阳性细胞平均值为39.50 ± 2.52;这些结果可能反映了死亡前的慢性缺氧条件，因为ORP-150仅在缺氧条件下诱导，而不是急性缺氧。一个 ...更多信息 慢性缺氧状态可能是SIDS的先兆。因此，OPR-150在SID病例脑中的免疫组化分析可能对鉴别SIDS和急性窒息非常有用。此外，我们还检测了ORP-150在成人标本中的表达，并探讨了其在法医学实践中的意义。本文报告31例（窒息9例，低温4例，失血5例，CO中毒6例，心源性猝死7例）脑皮质标本。采用相同的免疫组化方法。在多元线性回归方法中，估计的ORP-150对年龄的回归系数是显著的（p0.039），因此，我们可以发现ORP-150表达水平依赖于年龄。采用协方差分析，比较各死因ORP-150、LS_（1VIEAN）的均值，LS_（1VIEAN）是排除年龄影响的假设平均值。LSMEAN +/-S.E.低体温组为84.74 ± 9.03，窒息组为57.52 ± 6.34，CO组为46.68 ± 6.70，放血组为24.84 ± 8.05，SCD组为16.24 ± 7.35。分析的结果是，ORP-150表达水平的LSMEAN与死亡原因相关。死亡前脑缺血的持续时间可能存在差异。例如，SCD被认为是即时死亡，而脑缺血在窒息、CO和失血中持续几分钟，在体温过低的情况下持续几小时。因此，在法医尸检中，对脑组织中ORP-150的免疫组织化学和形态计量学分析可能有助于确定脑缺血的持续时间。少

The oxygen regulated protein 150-kDa (ORP-150) is only induced in hypoxic conditions. We performed an immunohistochemical and morphometrical study on the expression of ORP-150 in the brains of sudden infant death (SID) victims. The cerebral cortexes of 18 infants were used for this study. Each tissue section was incubated with anti-ORP-150 polyclonal antibodies and the number of ORP-150 positive cells was counted. In the cluster analysis, the 18 cases were classified into three groups (A-C groups). Group A was composed of 6 sudden infant death syndrome (SIDS) cases and its mean value of ORP-150 positive cells was 66.75+/-3.44, Group B (6 severe respiratory infectious disease such as pneumonia and bronchitis including sepsis) : 39.50+/-2.52 and Group C (5 SIDS and 1 severe respiratory infectious disease) 16.00+/-2.92, respectively. These results might reflect chronic hypoxic condition before death, because ORP-150 is only induced when a hypoxic condition exist, but not acute hypoxia. An … More d chronic hypoxic state is likely to be antecedent to SIDS. Therefore, immunohistochemical analysis of OPR-150 in the brain of SID cases may be very useful to differentiate between SIDS and acute asphyxia. Moreover, we examined the expression of ORP-150 in the adult samples, and discussed its significance in forensic practice. The cerebral cortexes of 31 cases (asphyxia 9 cases, hypothermia : 4, exsanguinations 5, CO intoxication (CO) : 6, sudden cardiac death (SCD): 7) were used for this study. The same immunohistolical method was used. In the multiple linear regression method, the estimated regression coefficient of ORP-150 on age was significant (p0.039), thus, we could find that the ORP-150 expression level depended on age. Using analysis of covariance, we compared the means of ORP-150, LS1VIEAN, which means hypothetic average value excluding influence of age, for each cause of death. The LSMEAN +/-S.E. was 84.74+/-9.03 in hypothermia, 57.52+/-6.34 in asphyxia, 46.68+/-6.70 in CO, 24.84+/-8.05 in exsanguinations, and 16.24+/-7.35 in SCD. As a result of the analysis, the LSMEAN of the ORP-150 expression level was related to the cause of death. There might be differences in the duration of brain ischemia before death. For example, SCD is presumed to be instant death, while brain ischemia continues for several minutes in asphyxia, CO and exsanguinations, and for several hours in hypothermia cases. Therefore, the immunohistochemical and morphometrical analysis of ORP-150 in the brain may be very useful to determine the duration of brain ischemia before death in forensic autopsy cases. Less