Production of Oxidative Stress-Related ProteinChip and Its Evaluation on Biomarker for Common Disease

为了确定血清蛋白质作为疾病预防研究的生物标志物，直到2005年3月底，以下基础种子已经开发出来：1)使用SELDI-TOF-MS、IS-MS和二维电泳法对蛋白质组进行全球综合分析，以及2)开发Robot系统来准备用于蛋白质组分析的样本，以便对许多临床样本进行高通量分析。通过这些方法，本项目的研究人员能够使用它们所使用的实验和临床疾病模型来分析血清蛋白质组，如糖尿病、动脉粥样硬化、缺血再灌注损伤、癌症和过敏性疾病。此外，我们还进行了制备和开发一种新型的蛋白质芯片的初步研究，在芯片上放置了抗8-羟基脱氧鸟苷等单抗。然而，电喷雾沉积方法不足以将其沉积在芯片上。

In order to determinate serum proteins as a biomarker for disease prevention study, the following fundamental seeds were developed until the end of March in 2005 ; 1)global comprehensive analysis for proteome using SELDI-TOF-MS, IS-MS, and two-dimentional elctrophoresis, and 2)the development of Robot System to prepare the sample for proteome analysis to perform a high-through-put analysis for many clinical samples.By these methodology, investigators in this project are able to analyze the serum proteome using their used experimental and clinical disease models, such as diabetes mellitus, atherosclerosis, ischemia-reperfusion injury, cancer, and allergic diseases. In addition, we performed a preliminary study to make and to develop a novel protein chip on which monoclonal antibody such as anti-8-hydroxydeoxyguanosine monoclonal antibody is putted. However, the electrospray deposition method was not enough to deposit it on the chip.