Cell transplantation therapy for hemiplegia after cerebral hemorrhage

我们建立了一种新的大鼠自发性脑出血模型。该模型可经脑内诱导。注射IV型胶原酶，纹状体出血，模拟人脑出血。甲基苯丙胺致动物偏瘫，运动和特定旋转运动减少。旋转与出血量相关。组织学分析显示病变侧黑质有巨噬细胞/小胶质细胞浸润，随后出现黑质变性。原位杂交研究表明，损伤侧黑质中Na/肌醇共转运蛋白上调。病变侧黑质变性可能与甲基苯丙胺引起的旋转有关。脑源性神经营养因子（BDNF）在出血周围纹状体中表达，为神经干细胞移植提供了条件。因此，我们对该出血模型进行了干细胞移植研究，但与胎儿组织移植相比，移植细胞率较低。我们计划将基因转移到干细胞，但是我们无法在这个学期完成这个项目。

We have developed a new model of rat spontaneous intracerebral hemorrhage. The model can be induced by intracerebra]. injection of collagenase type IV with which hemorrgae occurred in the striatum and mimicked the human intracerebral hemorrhage. The animal developed hemiplegia, reduced motion and specific rotation movement induced by methamphetamine. The rotation was correlated with amount of hemorrhage. Histological analysis indicated infiltration of macrophage/microglia in the lesion-side substantia nigra, which is followed by degeneration of the substantia nigra. In situ hybridization study indicated upregulation of Na/myoinositol cotransporter in the lesion-side substantia nigra. Degeneration of the lesion-side substantia nigra might be related to the methamphetamine-induced rotation. The brain-derived neurotrophic factor (BDNF) was indiced in the perihemorrhage striatum providing suitable condition for transplantation of the neural stem cell. We, therefore developed stem-cell transplantation study for this hemorrhage model, but transplanted-cell rate was rather low as compared to the fetal tissue transplant. We planned gene transfer to stem cell, yet we could not finish the project in this term.