Chemical tools for analysis and manipulation of ceramidases in human disease

神经酰胺酶是一种分解信号脂神经酰胺的酶。它们在许多生理和病理过程中发挥着重要作用。然而，它们的活性很难分析，几乎没有任何抑制剂作为生化工具，甚至作为药理候选药物。在这个项目中，针对单个神经酰胺酶的特定探针将被开发和验证，并用于开发这些酶的抑制剂。将测试这些探针用于流式细胞术的适用性，并将开发适当的方法来简单而可靠地分析这些活动，以显著推进这一研究课题。

Ceramidases are enzymes that cleave the signal lipid ceramide. They play an important role in numerous physiological and pathological processes. However, their activity is difficult to analyse and there are hardly any inhibitors as biochemical tools or even as pharmacological drug candidates. In this project specific probes for individual ceramidases will be developed and validated and used to develop inhibitors for these enzymes. The suitability of these probes for flow cytometry will be tested and appropriate methods for a simple and reliable analysis of these activities will be developed in order to advance this subject of research in a significant way.