RESEARCH ON PREDICTION OF PERIODONTAL DISEASE IN CHILDREN AND ADOLESCENTS

牙周炎的发病机制通常被认为是由特定的细菌引起的，然而，儿童和青少年牙周特定细菌的发生率仍然未知。在本研究中，10个假定的牙周致病菌进行了纵向检查，从119个牙周健康儿童（2 - 15岁）的菌斑和唾液样本，使用聚合酶链反应方法。在唾液中经常发现赭色二氧化碳嗜纤维菌、生痰二氧化碳嗜纤维菌和伴放线杆菌，并且在本研究的其余时间中倾向于在唾液中检测到，而牙龈卟啉单胞菌、齿垢密螺旋体和中间普雷沃菌很少检测到。变黑普雷沃菌在菌斑中检出率较高，且患病率随年龄增长而增加。弯曲杆菌和腐蚀艾肯氏菌有时从菌斑和唾液中检测到，而拟杆菌在唾液中很少检测到。我们的研究结果表明，A. actinomycetemcomitans、伴放线菌C. ochracea，C. sputigena、黑腐霉（P. nigrescens）、C. rectus和E. corrodens是健康儿童口腔微生物植物群的常见成员，而牙龈卟啉单胞菌、中间卟啉单胞菌和T.齿垢似乎是短暂的生物体。

The pathogenesis of periodontitis is generally accepted to be initiated by specific bacteria, however, the occurrence rate of specific periodontal bacteria in children and adolescents remains unknown. In the present study, 10 putative periodontopathic bacteria were longitudinally examined in plaque and saliva samples from 119 periodontally healthy children (2 - 15 years old) using a polymerase chain reaction method. Capnocytophaga ochracea, Capnocytophaga sputigena, and Acrinobacillus actinomycetemcomitans were frequently found in saliva, and tended to be detected in saliva for the rest of the study, whereas Porphyromonas gingivalis, Treponema denticola, and Prevotella intermedia were rarely detected. Prevotella nigrescens was more frequently detected in plaque and its prevalence increased with age. Campylobacter rectus and Eikenella corrodens were sometimes detected from both plaque and saliva, while Bacteroides forsythus was less occasionally detected in saliva. Our findings suggest that A. actinomycetemcomitans, C. ochracea, C. sputigena, P. nigrescens, C. rectus, and E. corrodens are common members of the oral microbial flora of healthy children, whereas P. gingivalis, P. intermedia, and T. denticola appear to be transient organisms.