LC-ICP-MS/MS System

这项申请的主题是采购LC-ICPMS/MS系统，用于在超痕量范围内分析人体和环境样品中的痕量元素。申请者无法获得这项技术，可用的仪器在灵敏度、特异度和吞吐量方面不符合最先进的元素分析的要求。LC-ICPMS/MS系统能够在不受干扰的情况下检测元素及其物种，并对其进行分析。因此，它们非常适合于执行申请书中列出的项目，对于应用研究小组未来在微量元素研究方面的生存至关重要。上述研究领域之一涉及建立微量采样方法，用于血液和尿液中有毒金属的生物监测。微量采样只需要非常少量的基质(&lt；100微米L)，因此非常适合于大量样品的采集或储存存在问题的研究，例如在发展中国家。因此，必须满足对分析灵敏度的最高要求，以便即使在最低浓度下也能检测到痕量元素。此外，还将评估微量采样方法在形态分析中的适用性。这就需要将电感耦合等离子体质谱/质谱仪与色谱学结合起来，进一步的项目包括对生物样品中的多种元素进行测定，这些样品有时来自数千名测试者的研究。电感耦合等离子体质谱/质谱仪系统适合于高通量分析，因为它们可以一次测定几种元素，因此每个样品所需的时间相对较短。如果需要，可以在选定的样品中确定元素的种类，以详细评估毒理学相关性。另一个研究领域是微量元素在某些疾病中的影响。由于这部分是在细胞和小鼠模型中进行的，在这些模型中通常只有微量样品可用，因此这里也必须满足对微量元素分析的最高要求。总之，通过获得LC-ICP-MS/MS系统，参与应用的工作组可以更有效和高效地开展当前项目的工作，并对新的研究问题做出灵活反应。此外，他们还准备满足未来几年的要求。此外，联合使用该系统为工作组之间的合作开辟了新的机会。集成到LMU Klinikum的核心设施使所有感兴趣的研究小组都可以进行痕量元素分析。

The subject of this application is the procurement of an LC-ICP-MS/MS system for the analysis of trace elements in human and environmental samples in the ultra-trace range. Access to this technology is not available to the applicants and the instrumentation available does not meet the requirements of state-of-the-art elemental analysis in terms of sensitivity, specificity and throughput. LC-ICP-MS/MS systems are able to detect elements and their species down to the sub-ppt range and analyse them without interference. Thus, they are ideally suited for the execution of the projects listed in the application and are essential for the future viability of the applying research groups with regard to trace element research.The necessity to acquire an LC-ICP-MS/MS system is, among other things, due to the small amounts of analytes that have to be determined in different matrices. One of the research fields described above involves the establishment of microsampling methods for the biomonitoring of toxic metals in blood and urine. Microsampling requires only very small amounts of matrix (< 100 µl) and is therefore ideally used for studies where the collection of large amounts of sample or its storage is a problem, e.g. in developing countries. Consequently, highest demands must be met regarding the sensitivity of the analysis so that trace elements can be detected even at the lowest concentrations. Furthermore, the suitability of microsampling methods for speciation analysis will be evaluated. This requires the coupling of ICP-MS/MS with chromatography.Further projects include multi-element determinations in biosamples from studies with sometimes thousands of test persons. ICP-MS/MS systems are suitable for high throughput analysis because they can measure several elements in one determination and the time required per sample is therefore relatively low. If required, the species of the elements can be determined in selected samples to evaluate the toxicological relevance in detail. Another field of research is the influence of trace elements in certain diseases. As this is partly investigated in cell and mouse models, in which often only micro samples are available, the highest demands on trace element analysis must be met here as well.In summary, by acquiring an LC-ICP-MS/MS system, the working groups involved in the application can work on current projects more effectively and efficiently and react flexibly to new research questions. In addition, they are prepared to meet future requirements for years to come. Furthermore, the joint use of the system opens up new opportunities for collaborations between the working groups. The integration into the core facility of the LMU Klinikum makes trace element analysis accessible to all interested research groups.