Study on the development of the electrochemical screening for endocrine disruptors

在研究了蛋白质与其配体亲和素和生物素相互作用的基础上，建立了一种新的内分泌干扰物（EDCs）的电化学筛选方法。为了评价内分泌干扰物与雌激素受体（EK）的结合能力，制备了柔红霉素标记的17 β-雌二醇电活性化合物。与柔红霉素相比，所制备的标记雌二醇（LE）具有较高的电化学灵敏度。LE和ER之间的相互作用被观察到的LE的电极响应的降低。发现这种降低是由于LE与ER的特异性结合。LE与17 β-雌二醇竞争ER上的限制性结合位点，使LE的峰电流增加。然后，可以从峰值电流的增加获得针对LE的竞争者的浓度。在1 x 10 μ M 17 β-雌二醇下的相对标准偏差约为10%（n = 7）。<-8>通过与17 β-雌二醇-雌激素受体相互作用的比较，评价了内分泌干扰物与雌激素受体的结合亲和力。双酚A、对-壬基苯酚和p，p，p'-DDT用作用于EDC的测试化合物。所有的测试化合物都表现出一定的能力与ER结合。这种电化学结合试验说明了一种新的方法来评估的结合能力，ER，而不需要一个程序的结合和游离LE。迁移系统的开发进行了毛细管电泳分离分析的内分泌干扰物使用硫酸化β-环糊精。此外，还分别研究了SnO_2/Ti电极和碳纤维电极对水中内分泌干扰物的电分解和去除。

A new electrochemical screening method for endocrine disrupting chemicals(EDCs) was developed after the fundamental investigation of the interaction of a protein and its ligand, such as avidin and biotin. To evaluate the binding ability of EDCs to the estrogen receptor(EK), 17 β-estradiol labeled with daunomycin as an electroactive compound was prepared. The electrochemical sensitivity of the prepared labeled estradiol(LE) was high, as compared with daunomycin. The interaction between LE and ER was observed by the decrease in the electrode response of LE. This decrease was found to be due to the specific binding of LE with ER. The competitive reaction between LE and 17β-estradiol for the limiting binding sites on ER produced increase in the peak current of LE. Then the concentration of competitors against LE can be obtained from the increase in the peak current. The relative standard deviation at 1 x 10^<-8> M 17 β-estradiol was about 10 % (n=7). The binding affinity between EDCs and ER were also evaluated by comparison with 17β-estradiol-ER interaction. Bisphenol A, p-nonylphenol and p,p,p'-DDT were used as a test compound for EDCs. All test compounds demonstrated some ability to bind with ER.This electrochemical binding assay illustrates a new method for evaluating the binding capacity of EDCs to ER without the need for a separation procedure for the bound and free LE.The development of a migrating system for the separation analysis of EDCs was performed by capillary electrophoresis using sulfated β-cyclodextrin. Moreover, the electrochemical decomposition and removal of EDCs in water was investigated using SnO_2/Ti and carbon fiber electrode, respectively.