Study of the improvement in the resolution of capillary electrophoresis using affinity interaction

利用亲和相互作用提高毛细管电泳分辨率的研究

• 批准号: 05640673
• 负责人: TANAKA Shunitz
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640673/
• 关键词: Affinity interaction; Capillary electrophoresis; Avidin-biotin; Humic acid; Niacin derivatives; ヘミミセル; 電気化学検出器; アビジンとビオチン; ベースペアリング; 陽イオン界面活性剤

The aim of this project is to improve the resolution of capillary electrophoresis (CE) by means of a new mechanism for separation, especially, the introduction of affinity interaction in a capillary.For the achievement of the object, the interaction between various materials was investigated electrochemically and electrophoretically.The carbon paste electrode modified with copper (II)-cyclohexylbutyrate has a function for the accumulation of amino acids and lipoic acid as a metal complex and for the electrochemical datection of them sensitively.Sugars can be selectively accumutated on the electrode modified with copper (II)-porphyrin and detected electrochemically.The avidin-bitin interaction was investigated as the model of the interaction between protein and the ligand.Using the labeled biotin with electroactive compound such as daunomycin, the electrode response of the electroactive part decreased by the specific binding with avidin.The probability in the development of electrochemi … More cal assay for biotin was shown on the sasic of the competition reaction between the labeled biotin and biotin.The interaction between humic acid and heavy metal ions was also investigated.It was found that the complexing ability of humic acid increased with increasing ionic strength.In CE,the interaction with the hemimicelle formed on the surface of a capillary improved the resolution in the separation of organic anions.The separation of niacin derivatives was investigated on the basis of the acid dissociation equilibrium and the distribution equilibrium into sodium dodecyl surfate (SDS).At low pH (2.8), thirteen niacin derivatives were sepaated completely although long analytical time was required.By micellar electrokinetic chromatography, the complete separation of 13 niacin derivatives was achieved within 20 min.Further, the distribution coefficients of them into SDS micelle were evaluated.It was demonstrated that the introduction of affinity interaction in a capillary would offer the improvement in the resolution of CE. Less

本课题的目的是通过引入一种新的分离机制，特别是在毛细管中引入亲和作用，来提高毛细管电泳的分离度，为实现这一目标，用电化学和电化学方法研究了各种材料之间的相互作用，用铜（II）-环己基丁酸作为金属络合物具有富集氨基酸和硫辛酸的作用，并能灵敏地电化学检测它们.糖在铜（Ⅱ）-卟啉修饰电极上能选择性地被富集并电化学检测.亲和素-以生物素与配体的相互作用为模型，研究了生物素与配体的相互作用.用道诺霉素等电活性化合物标记生物素，由于与亲和素的特异性结合，使电活性部分的电极响应降低，并探讨了生物素与配体发生电化学反应的可能性 ...更多信息 利用生物素与标记生物素之间的竞争反应，研究了腐殖酸与重金属离子的相互作用，发现腐殖酸的络合能力随离子强度的增加而增强，在CE中，与毛细管表面形成的半胶束相互作用提高了有机阴离子的分离度根据酸解离平衡和在十二烷基硫酸钠（SDS）中的分配平衡，研究了十二烷基硫酸钠衍生物在低pH下的酸解离平衡（2.8）中的烟酸衍生物，虽然分析时间较长，但13种烟酸衍生物均得到了完全分离，用胶束电动色谱在20 min内实现了13种烟酸衍生物的完全分离。计算了它们在SDS胶束中的分配系数，表明毛细管中引入亲和作用可提高毛细管电泳的分离度。少

项目成果

K. Sugawara,: "Electrochemical assay of avidin and biotin using a biotin derivative labeled with an electroactive compound." Anal. Chem.67. 299-302 (1995)

K. Sukawara，：“使用用电活性化合物标记的生物素衍生物对抗生物素蛋白和生物素进行电化学测定。”

K.Sugawara: "Electroanalysis of lipoic acid using a carbon paste electrode modified with nickel (II)-cyclohexylbutyrate." J.Electroanal.Chem.347. 393-398 (1993)

K.Sukawara：“使用用环己基丁酸镍 (II) 修饰的碳糊电极对硫辛酸进行电化学分析。”

T.Kaneta,: "Effect of cetyltrimethylammonium chloride on electro-osmotic and electrophoretic mobilities in capillary zone electrophoresis," J.Chromatogr.,. 653. 313-319 (1993)

T.Kaneta，：“十六烷基三甲基氯化铵对毛细管区带电泳中电渗和电泳迁移率的影响”，J.Chromatogr.，。

K.Sugawara,: "Electrochemical behavior of sugar by using a carbon paste electrode modified with copper(II)-porphyrin," J.Electroanal.Chem.,. (in press.). (1995)

K.Sukawara，：“使用铜（II）-卟啉修饰的碳糊电极观察糖的电化学行为，”J.Electroanal.Chem.,。

K.Sugawara,: "Determination of amino acids by a chemically modified carbon-paste electrode with copper(II)-cyclohexylbutyrate," Bioelectrochemistry and Bioenergetics,. 31. 229-234 (1993)

K.Sukawara，：“通过化学修饰的碳糊电极与铜（II）-环己基丁酸测定氨基酸”，生物电化学和生物能量学，。