Distribution of natural and synthetic estrogens and some factors in aquatic environments of dairy area

本研究旨在探讨天然雌激素和人工雌激素在奶牛场水环境中的分布及其毒性的影响因素。因此，(1)研究了雌激素敏感和选择性分析方法的发展，(2)对乳制品和城市地区河水中雌激素污染的调查，(3)开发了水中雌激素的去除方法，并获得了以下结果。(1)以生物素化雌二醇为探针配体，建立了抗原抗体免疫反应与鸟苷生物素技术相结合的17 β-雌二醇ELISA检测方法。用碳纳米管修饰电极建立了一种电化学检测方法。此外，DES与电活性凹槽粘合剂在电极上光固定DNA结合位点的竞争反应表明了检测合成雌激素之一DES的可能性。(2)采用酶联免疫吸附法（ELISA）对北海道北部北开市周边奶牛场西别河的17种β雌激素二醇和总雌激素的分布进行了调查。在这条河的所有采样点都发现了雌激素的微摩尔水平。由于总雌激素值与17 β -雌二醇浓度接近，因此认为大多数雌激素是雌二醇。(3)采用碳纤维电极对雌二醇和苯胺进行电化学氧化粘附，海藻酸盐凝胶珠含活性炭，开发了对部分有害有机物的去除方法。

The aims of this study are to investigate the distribution of natural and synthetic estrogens and some effecting factors on the toxicy in aquatic environments of dairy area. Therefore, (1) the development of sensitive and selective analytical methods for estrogens, (2) the investigation of pollution with estrogens in river water of dairy and urban area, and (3) the development of removal methods for estrogens in water, were studied and the following results were obtained.(1)An ELISA method detecting 17 β-estradiol sensitively and selectively was developed by using biotinylated estradiol as a probe ligand, which can combine immuno-reaction of antigen and antibody with avidine-biotin technologies. An electrochemical detecting method was also established with the modified electrode with carbon nano-tube. Moreover, the possibility of detecting DES, one of synthetic estrogens was shown by a competition reaction of DES with an electroactive groove binder at the binding sites of photo-immobilized DNA on the electrode.(2)The distribution of estrogens in water of Nishibetsu river, which flows in dairy area around Bekkai city in northern area of Hokkaido Prefecture, was investigated by the ELISA methods for 17 βestrodiol and total estrogens. The presence of pico molar level of estrogens in all sampling points of this river was found out. Since the values of total estrogens and 17 β -estrodiol concentration are close, it is thought that most of estorgens is estradiol.(3)The removal methods for some harmful organic compounds were developed by carbon fiber electrode, where estradiol and aniline were oxidized and adhered electrochemically, and alginate gel beads containing activated carbon.