Distribution of natural and synthetic estrogens and some factors in aquatic environments of dairy area

奶牛区水生环境中天然和合成雌激素的分布及相关因素

• 批准号: 17550068
• 负责人: TANAKA Shunitz
• 金额: $ 2.18万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17550068/
• 关键词: 17 β-estradiol; estrogens; dairy area; ELISA; Nishibetsu river; イムノアッセイ; 電気化学検出

The aims of this study are to investigate the distribution of natural and synthetic estrogens and some effecting factors on the toxicy in aquatic environments of dairy area. Therefore, (1) the development of sensitive and selective analytical methods for estrogens, (2) the investigation of pollution with estrogens in river water of dairy and urban area, and (3) the development of removal methods for estrogens in water, were studied and the following results were obtained.(1)An ELISA method detecting 17 β-estradiol sensitively and selectively was developed by using biotinylated estradiol as a probe ligand, which can combine immuno-reaction of antigen and antibody with avidine-biotin technologies. An electrochemical detecting method was also established with the modified electrode with carbon nano-tube. Moreover, the possibility of detecting DES, one of synthetic estrogens was shown by a competition reaction of DES with an electroactive groove binder at the binding sites of photo-immobilized DNA on the electrode.(2)The distribution of estrogens in water of Nishibetsu river, which flows in dairy area around Bekkai city in northern area of Hokkaido Prefecture, was investigated by the ELISA methods for 17 βestrodiol and total estrogens. The presence of pico molar level of estrogens in all sampling points of this river was found out. Since the values of total estrogens and 17 β -estrodiol concentration are close, it is thought that most of estorgens is estradiol.(3)The removal methods for some harmful organic compounds were developed by carbon fiber electrode, where estradiol and aniline were oxidized and adhered electrochemically, and alginate gel beads containing activated carbon.

本研究旨在探讨天然雌激素和合成雌激素在奶牛场水环境中的分布及其毒性的影响因素。为此，本论文开展了(1)雌激素灵敏、选择性分析方法的研究，(2)乳牛场及城市河水中雌激素污染的调查研究，(3)水中雌激素去除方法的研究，取得了以下结果：(1)以生物素标记的雌二醇为探针配体，建立了一种将抗原、抗体免疫反应与亲和素-生物素技术相结合的检测17-β-雌二醇的ELISA方法。并建立了碳纳米管修饰电极的电化学检测方法。此外，通过DES与电极上光固定DNA结合部位的电活性沟槽结合部位的竞争反应，证明了检测合成雌激素DES的可能性。(2)采用17β雌二醇和总雌激素的酶联免疫吸附试验方法，研究了流经北海道北部北开市周边奶牛场的西北江水中雌激素的分布。在这条河的所有采样点中都发现了雌激素皮摩尔水平的存在。由于总雌激素值和17β-雌二醇浓度接近，因此认为雌激素主要是雌二醇。(3)利用碳纤维电极氧化雌二醇和苯胺，并结合含有活性碳的海藻酸凝胶小球，建立了一些有害有机物的去除方法。

项目成果

Voltammetric Behavior and Determination of 17β-Estradiol at Multi-Wall Carbon Nanotube-Nafion Modified Glassy Carbon Electrode

多壁碳纳米管-Nafion 修饰玻碳电极上的伏安行为及 17β-雌二醇的测定

• 发表时间: 2006
• 期刊: Analytical Science 22
• 作者: S.Tanaka (Graduate School of Environmental Science;Hokkaido University);Y.Matsumoto;H.Tsue;Y.Matsumoto;N.Terui
• 通讯作者: N.Terui

Removal of organic compounds by alginate gel beads with entrapped activated carbon.

• DOI: 10.1016/j.jhazmat.2005.01.010
• 发表时间: 2005-04
• 期刊: Journal of hazardous materials
• 影响因子: 13.6
• 作者: Yongbo Lin;Bunshi Fugetsu;Norifumi Terui;Shunitz Tanaka

Science and technology for environmental remediation

环境修复科学技术

• 发表时间: 2007
• 期刊: Hokkaido University Publisher
• 作者: S.Tanaka (Graduate School of Environmental Science;Hokkaido University)
• 通讯作者: Hokkaido University)

水の分析第5版(日本分析化学会北海道支部編)

水分析第5版（日本分析化学会北海道分会编）

• 发表时间: 2005
• 作者: H.Tsue;T.Takimoto;C.Kikuchi;H.Yanase;H.Takahashi;K.Amezawa;K.Ishibashi;S.Tanaka;R.Tamura;田中俊逸(北海道大学大学院環境科学院編);田中俊逸;田中俊逸(日本分析化学会北海道支部編);田中俊逸
• 通讯作者: 田中俊逸

Electrochemical oxidation for low concentration of aniline in neutral pH medium: Application to the removal of aniline based on the electrochemical polymerization on a carbon fiber

• DOI: 10.1021/es040379f
• 发表时间: 2005-05-15
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY
• 影响因子: 11.4
• 作者: Matsushita, M;Kuramitz, H;Tanaka, S
• 通讯作者: Tanaka, S