Hunt for early diverged stramenopiles and construction of taxonomic system

在研究期间，获得了以下结果：1。发现了两个未描述的异养层虫，并作为两个新属（Wobblia和Placidea）报道。分子分析表明，这些生物在异源异色植物出现之前就已经分化了，并且它们形成了一个独立于任何已知的叠层纲分支的分支。提出了一个新纲——Placididea或Placidiophyceae。提出了异源异源生植物Pinguiophyceae的新分类。这一类具有生物化学特征：迄今已知的所有成员都能产生丰富的omega - 3脂肪酸。非光合叠层动物，盘藻和纤毛藻，被发现有叶绿体编码基因rbcL。这个基因几乎是完整的，不太可能是假基因。在翼单胞菌中，用电子显微镜证实了退化叶绿体的存在。利用电子显微镜和分子数据（18S rDNA和rbcL）对三目双齿藻进行了研究。基于形态学（LM）和行为学（EM）的分类特征，对已有文献中的分类特征进行了重新评价，并考虑了属的诊断特征。提出了花梗属分类的实用指南。发现并研究了一种变形虫藻类，推测它是双齿藻纲的原始成员。这种生物形成了一个分支，与被描述为无尾草的硫溶酶形成了一个分支。目前正在审议一项关于建立新秩序的建议。发现了一种在生命周期中产生双鞭毛虫游动细胞的变形虫藻类。与其他双鞭毛纲藻类的比较表明，该纲的原始形态为双鞭毛，具有典型的叠层鞭毛器官。所获得的结果允许重建特征进化可能发生在层桩的主要分支。

The following results were obtained during the period of this research :1. Undescribed two heterotrophic stramenopiles were discovered and reported as two new genera, Wobblia and Placidea. Molecular analyzes indicated these organisms diverged before emergence of the heterokont chromophytes, and that they form a clade independent from any known clades of the stramenopiles. A new class Placididea or Placidiophyceae was proposed.2. A new class of heterokont chromophytes, Pinguiophyceae, was proposed. This class is biochemically characterized : all the members so far known produce rich omega 3 fatty acids.3. Non-photosynthetic stramenopiles, Ptendomonas and Ciliophrys, were revealed to have chloroplast encoded gene rbcL. The gene was almost intact and unlikely a pseudogene. In Pteridomonas, the presence of vestigial chloroplast was confirmed with the electron microscope.4. Three orders of the class Dictyochophyceae were investigated with EM and molecular data (18S rDNA and rbcL). Taxonomic characters appeared in previously published articles were reevaluated based on cladistics of using morphological (LM and EM) and behavioral characters, and diagnostic characters of genera were considered. Practical guide for classification of Pedinellales genera was proposed.5. An amoeboid alga presumably a primitive member of the class Dictyochophyceae was discovered and examined. This organism formed a clade with Sulcochrysis described as incertae cedis. A proposal of a new order Sulcochrysidaies is under process.6. An amoeboid alga that produce biflagellate swimming cells in the life cycle was discovered. Comparison of this alga with other members of the Dictyochophyceae suggested that primitive state of the class was biflagellate and possession of typical stramenopile flagellar apparatus.The results obtained allowed to reconstruct character evolution presumably occurred in major clades of the stramenopiles.