Basal construction studies for the diversity of algae-protist complexes

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Understanding the dynamics of establishment, maintenance and collapse of the diversity of eukaryotic microorganisms in aquatic ecosystems, and the interaction among those organisms and interrelation between the organisms and environmental organic matter, are important issues of aquatic microbial biology. To evaluate factors that determine these dynamics, we conducted analyses of metabolomics, metagenomics and chlorophyll derivatives, along with microscopic observations. We identified some organic matter whose quantities increased or decreased coupled with the spatio-temporal changes of the biome. We also found a metabolite that can be a new biomarker to detect the predatory activity of protists. Furthermore, algae and protists requiring taxonomic re-evaluation were examined using established cultures.