Development of detection system by using infection of Cryptosporidium purvum oocyst to cultured human cell line

隐孢子虫是一种通过饮用水传播的传染性微生物。对于浓度较低的病原菌，有一些检测方法，如去孢子计数法等。本研究以细胞培养物为载体组织，建立了一种更为简便的细胞培养体系。该细胞培养自人小肠癌细胞HCT-8。隐孢子虫可以感染经ELISA方法检测到的生物体，作为一种消毒的目的，我们将其感染潜力集中在细胞上。活细胞和固定细胞培养显示感染细胞与正常细胞的差异。紫外线被认为是有效的，以减少感染的可能性的有机体。UV的强度为20 mW sec/cm ^2。

Cryptosporidium purvum is infectious microbes thorough drinking water. There are some detection methods for its low concentration such as de-spored counting. In this research, to develop more convenient system, the cell culture was applied as a carrier tissue. The cell was cultured from human small intestine cancer cell, HCT-8. Cryptosporidium could infect that was detected by ELISA method.As a purpose of disinfection of the organism, we focused its infectious potential to the cells. Living-cells and Fixed-cell culture showed the difference between infection and normal cell. Ultra violet was found to be effective to decrease the infectious potential of the organism. The intensity of the UV was 20 mW sec/cm^2.