The study of neuropathogenesis in the developing brain disorders induced by viral infection

我们研究了小鼠巨细胞病毒（MCMV）感染对发育中的小鼠大脑易感细胞和年龄相关的抵抗力的影响。将lacZ基因插入到晚期基因中的重组MCMV感染不同发育阶段的小鼠脑片。脑室区和皮质边缘区是MCMV感染的易感部位，随着脑的发育，易感性逐渐降低。免疫组化染色显示病毒易感细胞呈GFAP、nestin和Musashi-1阳性，提示VZ中的易感细胞为胶质祖细胞。这些结果表明，发育中的大脑中的未成熟的胶质细胞的数量可能与大脑的易感性CMV感染human.We研究了潜伏的小鼠CMV（MCMV）感染的小鼠大脑中的再激活，通过他们的转移到脑切片培养。我们用重组MCMV脑内感染新生和年轻成年小鼠。在感染后6个月取出大脑，并用于制备脑切片，然后培养长达4周。X-Gal阳性细胞见于脑边缘区或脑室壁未成熟神经细胞。免疫组织化学染色显示，X-Gal阳性的再活化细胞为神经干/祖细胞。这些结果表明，大脑疾病可能发生感染后很长一段时间内重新激活潜伏感染的未成熟的神经细胞。

We investigated the effects of murine cytomegalovirus (MCMV) infection on the developing mouse brain in terms of susceptible cells and age-related resistance. Mouse brain slices at different development stages were infected with recombinant MCMV in which the lacZ gene was inserted into a late gene. The ventricular zone (VZ) and cortical marginal region were the sites most susceptible to MCMV infection, and the susceptibility declined with the development of the brain. Immunohistochemical staining showed that the virus-susceptible cells were positive for GFAP, nestin and Musashi-1, suggesting the susceptible cells in the VZ are glial progenitor cells. These results suggest that the amount of immature glial cells in the developing brain may be closely associated with the susceptibility of the brain to CMV infection in humans.We examined the reactivation of latent murine CMV (MCMV) infection in the mouse brain by their transfer to brain slice culture. We infected neonatal and young adult mice intracerebrally with recombinant MCMV. The brains were removed 6 months after infection, and used to prepare brain slices that were then cultured for up to 4 weeks. X-Gal-positive cells were observed in marginal regions of the brains or immature neural cells in the ventricular walls. Immunohistochemical staining showed that the X-Gal-positive reactivated cells were neural stem/progenitor cells. These results suggest that brain disorders may occur long after infection by reactivation of latent infection in the immature neural cells.