Differential diagnosis and vaccine development of tick-borne encephalitis

通过流行病学研究确定蜱传脑炎（TBE）流行区，评价北海道株TBE病毒的致病性，检验欧洲TBE疫苗对北海道株TBE病毒的防治效果。我们还对北海道病毒株进行了系统发育研究，以确定其起源。结果表明，犬、马血清抗体阳性，表明该病毒主要分布在北海道南部地区。从病毒分离结果中发现北海道地区流行性乙型脑炎病毒的宿主动物为红绒螯虾和斑姬鼠。北海道毒株具有与其他毒株相同的致病性。北海道毒株在几百年前从远东的祖先病毒分化而来。在俄罗斯西伯利亚地区发现了TBE病毒的另一个亚型西伯利亚亚型。欧洲型TBE病毒疫苗对北海道、远东和西伯利亚的TBE病毒株均有效。制备了针对北海道株TBE病毒的单克隆抗体，可用于诊断。

We conducted epidemiological study to specify the endemic area of tick-borne encephalitis (TBE), evaluated the pathogenicity of TBE virus Hokkaido strain and tested the efficacy of European TBE vaccine to Hokkaido TBE virus strain. We also performed phylogenetic study of TBE virus Hokkaido strainsto estimate the origin.The results showed that TBE virus distribute in southern part of Hokkaido from the positive antibody results in dog and horse sera. Reservoir animals of TBE virus in Hokkaido were found to be Clethrionomys rufocanus and Apodemus speciosus from virus isolation results. TBE virus Hokkaido strains possessed common pathogenicity shared among TBE virus strains. TBE virus Hokkaido strain diverged from ancestor virus in Far East several hundred years ago. Another subtype of TBE virus, Siberian subtype was found to distribute in Siberian area of Russia. European type TBE virus vaccine was found to be efficacious to TBE virus Hokkaido, Far Eastern and Siberian strains. Monochlonal antibodies against TBE virus Hokkaido strain were produced and found to be useful for diagnosis.