Tissue reconstrudion with Ox vivo culturad auto logous mesenchymal stem cells

脂肪垫的自体移植在整形和重建手术中用于增加丢失的软组织有着悠久的历史。许多使用自体脂肪移植，珍珠和半液体脂肪组织在治疗凹陷的地区或疤痕在面部地区已被报道。尽管人们对改进游离脂肪自体移植的操作充满热情，然而，研究人员对移植物随时间推移而逐渐吸收感到失望。成脂肪细胞是脂肪细胞的单能前体细胞，其定向分化在胚胎发育过程中完成。未预期到脂肪母细胞的出生后供应。这就是为什么用于论证失去的脂肪组织的手术仅集中于移植预先形成的脂肪垫的原因。然而，我们最近在小鼠基底膜注射部位观察到的新生脂肪形成表明，在成年小鼠的结缔组织中分布着丰富的脂肪前体细胞群。 ...更多信息 并且它们可以迁移、增殖和成熟以形成具有所需尺寸和所需部位的脂肪垫。由于这一结果意味着一种增加失去的软组织的新技术，我们在此探索通过离体分离和培养这些脂肪细胞前体细胞作为用于脂肪垫增加的自体细胞来利用这些脂肪细胞前体细胞的可能性。我们分析了层粘连蛋白α4链G结构域的结构和功能，该结构域由脂肪前体细胞和内皮细胞合成，这两种细胞之间的基底膜结构的构建。在Toriyama和川口（2001）中，我们回顾了我们通过注射基质胶在小鼠中从头脂肪形成的成功。在Tajima等人（2001年）中，我们发现由于刺激的IV型胶原合成，3 T3-L1成纤维细胞在缺氧条件下加速脂肪转化。在后藤等人（2001）的研究中，我们利用链特异性RNA干扰技术分析了果蝇层粘连蛋白α、β、γ链的细胞内组装过程。在Kitagawa和Yamashita（2001）中，我们介绍了我们最近关于层粘连蛋白a链G结构域在外周结缔组织中的功能的数据。在Toriyama等人（2002）中，我们报道了用于再生软组织工程的脂肪细胞前体细胞的电子显微镜组织学。少

Auto-grafting of fat pads has a long history in plastic and reconstructive surgery for augmentation of lost soft tissue. Many uses of autogenous fat grafts, pearls and semi liquid adipose tissue in the treatment of depressed regions or scars in the facial area have been reported. Despite the enthusiasm for improved operation of free fat-autografts, however, researchers have been disappointed by progressive absorption of the graft with time. It has been accepted that the commitment of adipoblasts, unipotential precursor of adipocytes, completes during the embryonic development. Postnatal supply of adipoblasts has not been Expected. This is the reason why the surgery for argumentation of lost fat tissue has been focused only on transplantation of pre-formed fat pads. Our recent observation of de novo adipogenesis in mice at the site of injection of basement membrane, however, suggested that an abundant population of adipose precursor cells is distributed in connective tissues of adult bo … More dy and that they can migrate, proliferate and maturate to form : fat pads with desired size and at desired site. Since this result implies a novel technique of augmenting lost j soft tissue, we here explore for the possibility of taking advantage of such adipocyte precursor cells by isolating and culturing them ex vivo as the autogenous cell for fat pad augmentation.In Yamaguchi et al, (2000), we have analyzed the structure and function of the G domain of laminin α4 chain which is synthesized by both adipocyte precursor cells and endothelial cells for the construction of the basemenf membrane structure between these tow types of cells. In Toriyama and Kawaguchi (2001), we reviewed our success of de novo adipogenesis in mice by injection of Matrigel. In Tajima et al, (2001), we found accelerated adipose conversion of 3T3-L1 fibroblasts under hypoxia due to stimulated type IV collagen synthesis. In Goto et al, (2001), we analyzed intracellular process of the assembly of Drosophila laminin α, β.γ Chains taking advantage of chain-specific RNA interference. In Kitagawa and Yamashita (2001), we introduced our recent data on the function of laminin a chain G domain in the peripheral connective tissue. In Toriyama et al, (2002), we reported an electron-microscopic histology of the adipocyte precursor cells for regenerative soft-tissue engineering. Less