Preparation of non-biofouling blood bags

采用不同的水溶性聚合物对聚乙烯(PE)表面进行接枝改性，以控制血液/材料的相互作用。本研究通过光接枝聚合的方法，在低密度聚乙烯表面接枝了聚(2-甲基丙烯酰氧乙基磷酰胆碱)(PMPC)、聚(丙烯酰胺)(PAAM)、聚(N-乙烯基吡咯烷酮)(PVPy)、聚[甲基丙烯酰氧基聚乙二醇单甲酯](PmGMP)等水溶性聚合物，以加深对接枝到PE表面的水溶性聚合物化学结构对血小板功能影响的了解。用热处理法制备了水溶性聚合物改性PE袋和未改性PE袋，通过测定细胞内游离钙离子浓度([Ca^&lt；2+&gt；]i)来评价血小板在PE袋中保存后的活化情况。与PMPC接枝的PE表面接触的血小板[Ca~(2+)]_i与天然血小板水平相同，明显低于与其他PE表面接枝的水溶性聚合物的[Ca~(2+)]_i。与未处理的PE相比，PMPC和PmEg接枝的PE表面黏附的血小板数量明显减少。将富含血小板的血浆保存在PE袋中后，测定其聚集能力。接枝PMPC的PE表面的聚集能力明显低于未接枝的PE以及接枝PAAM、PVPy和PmEg的PE表面。我们的结论是，在这些水溶性聚合物中，PMPC的表面修饰在保存血小板功能方面是最有效的。

The surface of polyethylene (PE) was modified by grafting with various water-soluble polymers to control blood/materials interactions. This study was carried out in order to improve our understanding of the effect of chemical structure of water-soluble polymers grafted on the PE surface on platelet function when the platelets were in contacted the surface, Water-soluble polymers, such as poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), poly(acrylamide) (PAAm), poly(N-vinylpyrrolidone) (PVPy), and poly[monomethacryloyl poly(ethylene glycol)] (PMPEG) were grafted on low-density PE sheets by photoinduced graft polymerization. PE bags modified with water-soluble polymers and non-modified PE bags were prepared by heat processing.The activation of platelets after storage in the PE bags was evaluated by measuring the cytoplasmic free calcium ion concentration ([Ca^<2+>]i). The [Ca^<2+>]i of platelets in contact with the PE surface grafted with PMPC was the same level as that of native platelets and significantly less than that in contact with other PE surfaces grafted with water-soluble polymers. The number of adherent platelets was effectively decreased on PE surfaces grafted with PMPC and PMPEG compared with non-treated PE. The aggregation ability of platelets was also measured after storage of platelet-rich plasma in the PE bags. The PE surface grafted with PMPC demonstrated a significantly decrease in aggregation ability compared with the non-treated PE, and that grafted with PAAm, PVPy, and PMPEG. We conclude that surface modification with PMPC is the most effective among these water-soluble polymers in preserving platelet functions.