Structure biology for the causative genes of atopy and order-made therapy in the environment.

近年来，变态反应的致病基因被发现，致病分子（蛋白质）的结构被分析，并出现了序贯治疗。（1）IgE产生抑制系统及其缺陷：IL-12受体β2链基因突变已被证实是变态反应的致病基因之一。部分高IgE患者存在IL-12受体β2链基因突变。IL-12不能抑制患者外周血单核细胞的IgE产生。因此，这些突变可诱导高水平的IgE产生和过敏反应。其次，IL-18受体α2链基因突变已被确定为过敏的致病基因。部分高IgE患者存在IL-18受体α2链基因突变。IL-18不能抑制患者外周血单核细胞的IgE产生。因此，表明这些突变也诱导高水平的IgE产生和过敏反应。（2）致病基因的结构分析，并利用核磁共振技术对HAS蛋白的结构进行了分析。（3）序贯疗法的发展：我们还发表了用于临床或预防变态反应的特应性遗传分类和序贯疗法。

The causative genes of allergy have been newly found and the causative molecules (protein) have been structurally analyzed.Moreover, order-made therapy have been developed.(1)IgE production suppression systems and their defects.The mutations of IL-12 receptor β2 chain gene have been identified as one of the causative genes for allergy. Some patients with high levels of IgE production had the mutations of IL-12 receptor β2 chain gene. IL-12 was not able to inhibit IgE production of the patients' peripheral blood mononuclear cells. Therefore, it was indicated that these mutations induced high levels of IgE production and allergic reactions.Next, the mutations of IL-18 receptor α2 chain gene have been identified as the causative gene for allergy. Some patients with high levels of IgE production had the mutations of IL-18 receptor α2 chain gene. IL-18 was not able to inhibit IgE production of the patients' peripheral blood mononuclear cells. Therefore, it was indicated that these mutations also induced high levels of IgE production and allergic reactions.(2)Structural analysis of the causative genes.Moreover, we have published the information of structure of HAS protein by NMR.(3)Development of order-made therapy.Furthermore, we have published the genetical classification of atopy for clinical or prevention of allergy and order-made therapy have been published.