Study on suppression of interferon-γ production by nematode antigens

线虫抗原抑制干扰素-γ产生的研究

• 批准号: 14570218
• 负责人: UCHIKAWA Ryuichi
• 金额: $ 2.18万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570218/
• 关键词: nematode antigens; interferon-γ; lymphocytes; rats; mice; Nippostrongylus brasiliensis; Ascaris suum; ガンマ・インターフェロン; Nippostrongylus brasiliensis

In this project (2002-2003), we examine interferon (IFN)-γ production suppressing activity in excretory-secretory antigens (ES) derived from Nippostrongylus barasiliensis (Nb) and Ascaris suum (As) adult worms and pointed out the followings;1.Nb-ES antigen and its partially-purified fraction suppressed IFN-γ production by both rat and mouse T cells.2.Partially-purified As-ES also suppressed IFN-γ production by rat or mouse T cells, but the body fluid of the worm did not.3.After ion-exchange and anti-Nb-ES affinity chromatography, we obtained the Nb-ES fraction that has 20 times higher activity in suppression of IFN-γ production by rat T cells comparing with crude Nb-ES antigen.4.We also characterized the IFN-γ suppressing molecule(s) in Nb-ES.1)Molecular weight of the IFN-γ suppressing molecule(s) is 50-100kD.2)The molecule(s) is resistant to 56℃,30 min. but partially sensitive to 95℃,30 min treatment3)The molecule(s) is sensitive to periodate treatment.

在这个项目中（2002-2003），我们检测了来自巴氏日本圆线虫（Nippostronghylus barasiliensis，Nb）和猪蛔虫（Ascaris suum，As）成虫的排泄-分泌抗原（exclusive-secretory antigens，ES）中干扰素（IFN）-γ产生的抑制活性，并指出：1.Nb-ES抗原及其部分纯化组分抑制大鼠和小鼠T细胞产生IFN-γ。2.部分纯化的As-ES也抑制IFN-γ。3.经离子交换和抗Nb-ES亲和层析后，获得了抑制大鼠T细胞产生IFN-γ活性的Nb-ES组分，其活性是Nb-ES粗品的20倍。4.我们还对Nb-ES中的IFN-γ抑制分子进行了表征。1）IFN-γ抑制分子的分子量为50- 100 kD。2）该分子对56℃、30 min处理有抗性，但对95℃、30 min处理部分敏感。

项目成果

Kuroda A, Uchikawa R, Matsuda S, Yamada M, Tegoshi T, Arizono N.: "Up-regulation of Fas (CD95) and induction of apoptosis in intestinal epithelial cells by nematode-derived molecules."Infection and Immunity. 70. 4002-4008 (2002)

Kuroda A、Uchikawa R、Matsuda S、Yamada M、Tegoshi T、Arizono N.：“线虫衍生分子上调 Fas (CD95) 并诱导肠上皮细胞凋亡。”感染和免疫。

Kuroda, A.: "Up-regulation of Fas (CD95) and induction of apoptosis in intestinal epithelial cells by nematode-derived molecules"Infection and Immunity. 70. 4002-4008 (2002)

Kuroda, A.：“线虫衍生分子对 Fas (CD95) 的上调和肠上皮细胞凋亡的诱导”感染和免疫。