Study on suppression of interferon-γ production by nematode antigens

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In this project (2002-2003), we examine interferon (IFN)-γ production suppressing activity in excretory-secretory antigens (ES) derived from Nippostrongylus barasiliensis (Nb) and Ascaris suum (As) adult worms and pointed out the followings;1.Nb-ES antigen and its partially-purified fraction suppressed IFN-γ production by both rat and mouse T cells.2.Partially-purified As-ES also suppressed IFN-γ production by rat or mouse T cells, but the body fluid of the worm did not.3.After ion-exchange and anti-Nb-ES affinity chromatography, we obtained the Nb-ES fraction that has 20 times higher activity in suppression of IFN-γ production by rat T cells comparing with crude Nb-ES antigen.4.We also characterized the IFN-γ suppressing molecule(s) in Nb-ES.1)Molecular weight of the IFN-γ suppressing molecule(s) is 50-100kD.2)The molecule(s) is resistant to 56℃,30 min. but partially sensitive to 95℃,30 min treatment3)The molecule(s) is sensitive to periodate treatment.