Inhibitory mechanism of Interferon-γ production of peripheral blood mononuclear cells stimulated with OK-432 by soluble factor derived from oral cancer cells

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An in vivo simulation model was established of the immune status of patients with oral cancer by adding conditioned media (CM) derived from a healthy volunteer. PBMCs stimulated with OK-432 produced a large amount of IFN-γ ; however, both IFN-γ production and cytotoxic activity of PBMCs induced by OK-432 were inhibited by the addiction of CM. In order to examine these inhibitory effects against IFN-γ production, neutralization of inhibitory cytokines did not recover IFN-γ production induced by CM, suggesting the relation of unknown cytokines from the inhibition of IFN-γ production.Thus, the genes that regulated IFN-γ production were retrieved using cDNA microarray analysis. Some soluble factors included in CMs inhibited IFN-γ production in PBMCs by the suppression of IL-12 production via the inhibition of CD40 expression.