Clarification of transmission mechanism of urinary sensation in pontine micturition center and central effect of drugs for voiding dysfunction.

为了阐明脑桥排尿中心与排尿之间的关系，我们进行了电生理研究。在膀胱内插入导尿管，并注入生理盐水，直至引起自发收缩。通过小脑插入玻璃移液管微电极。记录了包括巴林顿核在内的中筋膜被盖的单一神经活动，同时观察了其与膀胱收缩有关的精确时间。神经元分为三种类型；那些只在收缩开始前放电的（E1），那些在收缩开始前不久开始并在收缩期间保持放电的（E2），以及那些在收缩期间强烈抑制放电的(I)。结果表明，诱导膀胱收缩的直接神经信号可能来自于巴林顿核。神经元分为三种类型，具有不同的功能作用。为了明确排尿功能障碍药物对排尿中枢的影响，在记录E1和E2神经元的同时，静脉注射抗胆碱能药物（盐酸奥昔布宁）和三环抗抑郁药物（盐酸氯米帕明）。随着奥昔布宁的使用，神经放电随着膀胱收缩的抑制而增加。另一方面，神经放电随着氯丙咪嗪的使用而减少。从这些结果来看，奥施布宁和氯丙咪嗪具有相似的外周作用和不同的中枢作用。为了明确去氨加压素对排尿中枢的影响，采用静脉和室内给药。在给药过程中，观察到神经元的放电随膀胱收缩的抑制而变化。从这些结果来看，去氨加压素可能起中心作用

We performed electrophysiological studies in order to clarify the relationship between the pontine micturition center and micturition. A catheter was inserted into the bladder and saline was injected until spontaneous contraction was induced. A glass pipette microelectrode was inserted through the cerebellum. Single neural activity was recorded in the mesopontine tegmentum including the Barrington's nucleus and simultaneously its precise timing in relation to bladder contraction was observed. Three types of neuron were distinguished ; those which fired only prior to the start of contraction (E1), those whose firing started shortly prior to and was maintained during contraction (E2), and those whose firing, was strongly suppressed during contraction (I). The results showed that direct neural signals to induce bladder contraction might arise from the Barrington's nucleus. The neurons were classified into three types with different functional roles.In order to clarify the effects of drugs for voiding dysfunction on the micturition center, an anticholinergic drug (oxybutynin hydrochloride) and a tricyclic antidepressant (clomipramine hydrochloride) were administered intravenously during the recording of E1 and E2 neurons. With the administration of oxybutynin, neural firing increased in accordance with the suppression of bladder contraction. On the other hand, neural firing decreased with the administration of clomipramine. From these results, it seemed that oxbutynin and clomipramine had similar peripheral effects and different central effects. In order to clarify the effects of desmopressin on the micturition center, it was administered intravenously and intraventriculary. With the administration, neurons were observed in which the firing changed in accordance with the suppression of bladder contraction. From these results, the possibility of the central effect of desmopressin was suggested