Expression and production of proteins containing in hemidesmosme/besement membrane at the interface between the implant-oral epithelium.

层粘连蛋白-5（Ln-5）与上皮细胞粘附和迁移相关，存在于结合上皮（JE）的内部和外部基底层（IBL和EBL）中，而Ln-5在种植体周围上皮（PIE）中的详细定位尚不清楚。通过免疫光学显微镜，Ln-5的免疫反应产物出现为沿着种植体-PIE界面的带，而界面的上部的大部分不表达Ln-5。Ln-5沿着与PIE、种植体周围沟上皮和口腔上皮的结缔组织界面表达。免疫电镜显示Ln-5在PIE的内3层、基底层及IBL、EBL中均有表达。其特征是仅在界面的下部形成100 nm宽的Ln-5阳性IBL和半桥粒（HDs）。此外，Ln-5阳性的EBL形成于大多数PIE-结缔组织界面，尽管在某些部分， ...更多信息 在界面处没有形成EBL和HDs。接下来，我们研究了PIE形成过程中Ln-5的分布。提取后一天，JE消失了。3天后，Ln-5阳性上皮细胞从口腔沟上皮（OSE）的前缘水平延伸到伤口上方。5天后，从伤口两侧的OSE延伸的上皮连接并形成额外的新上皮。新生上皮在BM中表达Ln-5。1 - 2周后，从伤口两侧延伸的口腔上皮在中心连接。植入后3天，无JE残留。Ln-5阳性上皮细胞从OSE顶部延伸。1 ~ 2周后，PIE形成。Ln-5在PIE-结缔组织界面表达。最后，4周后，Ln-5在种植体-PIE界面表达，PIE为非角化上皮。我们的研究结果表明，在PIE中IBL，EBL和HD的部分缺陷与Ln-5的存在有关，Ln-5是形成这些附着装置所必需的，并且与JE相比，由于附着装置的部分缺陷，PIE是粘附性差的渗透性上皮。此外，它表明，Ln-5诱导细胞迁移过程中PIE的形成，PIE起源于OSE。少

Laminin-5(Ln-5), which is associated with epithelial cell adhesion and migration, is present in the internal and external basement laminas(IBL and EBL) of the junctional epithelium(JE), whereas the detailed localization of Ln-5 in the peri-implant epithelium(PIE) remains unclear. By immuno-light microscopy, the immunoreactive product of Ln-5 appeared as a band along the implant-PIE interface, while most of the upper portion of the interface did not express Ln-5. Ln-5 was expressed along the connective tissue interfaces with the PIE, peri-implant sulcular epithelium, and oral epithelium. Immuno-electron microscopy showed that Ln-5 was expressed in the cells of the innermost-third layer and basal layer of the PIE, and in the IBL and EBL of the PIE. It was characteristic that a 100-nm-wide Ln-5-positive IBL and hemidesmosomes(HDs) were formed only in the lower portion of the interface. In addition, Ln-5-positive EBL was formed at most PIE-connective tissue interface, although at some part … More s of the interface, EBL and HDs were not formed. Next, we investigated the distribution of Ln-5 during the formation of PIE. One day after extraction, the JE had disappeared. After 3 days, Ln-5-positive epithelial cells extended horizontally over the wound at the leading edge from the oral sulcular epithelium(OSE). After 5 days, the epithelium extending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM. After 1 to 2 weeks, the oral epithelium extending from the sides of the wound joined in the center. Three days after implantation, no JE remained. Ln-5-positive epithelial cells extended apically from the OSE. After 1 to 2 weeks, the PIE was formed. Ln-5 was expressed at the PIE-connective tissue interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface, and the PIE was non-keratinized epithelium. Our findings suggest that partial deficiency of the IBL,EBL,and HDs in the PIE is associated with the presence of Ln-5, which is required for the formation of these attachment apparatuses, and that the PIE is a poorly adherent, permeable epithelium because of the partial deficiency of the attachment apparatuses, as compared to the JE. Furthermore it is suggested that Ln-5 induces cell migration during PIE formation, and that PIE originates from OSE. Less