Analysis of mechanism on enhancement of fibrinolysis as biological defense

老年人血栓形成的预防成为一个重要的问题。年龄似乎是血栓形成的大危险，我们检查了止血异常与年龄的关系。在维持性透析患者的检查中，随着年龄的增长，伴有血栓形成的死亡和危象频率增加。随着年龄的增长，主动脉出现钙化，动脉硬化进展。70岁以上健康志愿者的抗凝血酶和蛋白C水平降低，凝血酶-抗凝血酶复合物和可溶性纤维蛋白水平升高。而在这些老年人中，纤维蛋白原和纤维蛋白降解产物以及纤溶酶-纤溶酶抑制剂复合物水平升高，表明老年人存在高纤溶酶。老年健康人无继发血栓性纤溶。这些结果表明，纤维蛋白溶解系统的生理增强是由衰老引起的。纤溶调节系统中有纤溶酶原激活物（t-PA）、纤溶酶原激活物（PAI）、纤溶酶原激活物（PAI）、纤溶酶原激活物（PAI）、纤溶酶原激活物（PAI）和纤溶酶激活物（TAFI）。我们建立了ELISA测定总pai - 1、tPA- pai - 1复合物、TAFI和tPA在弥散性血管内凝血（DIG）、深静脉血栓形成（DVT）、多器官衰竭（MOF）和急性心肌梗死诊断中的有用性。随着老年人血浆中总pai - 1和TAFI水平的升高，这些因素可能在血栓形成中起重要作用。提示血浆pai - 1水平升高与pai - 1基因启动子多态性（4G/5G）有关。经知情同意后，检测老年人pai - 1基因（4G/5G）启动子多态性。年轻人和老年人的多态性分布无显著差异。我们的研究结果显示pai - 1基因启动子多态性与老年人血栓形成没有关系。最后，老年人血栓形成风险高，动脉粥样硬化明显，但处于高纤溶状态。少

The prevention of the thrombosis in the old people becomes an important problem. The aging seemed to be large risk of the thrombosis, and we examined the relation between hemostasis abnormality and aging. In the examination of the maintenance dialysis patient, the death and crisis frequency which accompanied thrombosis increased with the aging. With the aging, there were calcification of the aorta and progress of the arteriosclerosis. In more than 70 years old of healthy volunteer, antithrombin and protein C reduced and thrombin- antithrombin complex and soluble fibrin levels were increased. While, in those, fibrinogen and fibrin degradation products and plasmin- plasmin inhibitor complex levels were increased, suggesting that hyperfibrinolysis exist in old peoples. However old health peoples had no secondary fibrinolysis due to thrombosis. These results indicated that physiological enhancement in fibrinolytic system was caused by aging. There are plasminogen activator (t-PA), plasmino … More gen activator inhibitor (PAI)-I, II, III and thrombin activatable fibrinolysis inhibitor (TAFI) in regulatory system of fibrinolysis. We established the usefulness of measurement in total PAI-I, tPA-PAI-I complex, TAFI and tPA by ELISA for the diagnosis of disseminated intravascular coagulation (DIG), deep vein thrombosis (DVT), and multiple organ failure (MOF) and acute myocardial infarction. As plasma levels of total PAI-I and TAFI were increased in old peoples, these factors may play an important role of the onset in thrombosis. It was suggested that elevated plasma levels of PAI-I was related to the polymorphism of promoter in PAI-I gene (4G/5G). After informed consent, the polymorphism of promoter in PAI-I gene (4G/5G) was examined in old peoples.There was no significant difference of distribution in the polymorphism between young peoples and old peoples. Our results showed no relationship between polymorphism of PAI-I gene promoter and thrombosis in old peoples. Finally, old peoples had high risk for thrombosis and significant atherosclerosis, however they were in hyperfibrinolytic state. Less