Localization of high affinity choline transporter in the primate nervous system.

1.中枢神经系统的定位应用原位杂交和免疫组织化学技术，我们对高亲和力胆碱转运体（CHT）信使RNA和蛋白质在猕猴中枢神经系统的定位进行了研究。CHT mRNA和蛋白的信号定位于脑神经的运动核（躯体运动和副交感节前）、背外侧被盖核、臂旁核、脚桥被盖核、苍白球、新纹状体、延髓核、Broca斜角带核、Meynert基底核、内侧隔核和嗅结节中的神经元中。副交感节前神经元CHT mRNA和蛋白表达密度明显低于其他阳性神经元。在脊髓中，前角运动神经元显示CHT mRNA和蛋白质的信号。假定的γ-运动神经元总是表现出强烈的免疫反应CHT，而假定的α-运动神经元表现出 ...更多信息 各种免疫反应强度。在假定的α-运动神经元中，支配主背肌的神经元比支配肢体肌肉的神经元表现出更高的CHT含量。2.突触的超微结构定位为了确定CHT是否位于突触前膜或突触囊泡的膜，我们使用包埋后免疫细胞化学结合电镜分析了CHT在猴神经肌肉接头的定位。虽然免疫反应性弱于报告中的大鼠，大部分的CHT的免疫反应性位于神经末梢内，而不是在突触前膜。研究结果是兼容的CHT定位在水泡膜上的报告，在大鼠，但在水泡膜的准确定位，进一步的研究将是必要的，以确定最佳的条件，既保留了CHT的免疫反应性和结构的水泡膜。这些电子显微镜研究是与爱媛大学的Seiji Matsuda教授合作进行的。少

1.Localization in the central nervous systemUsing in situ hybridization and immunohistochemistry, we have determined the localization of high affinity choline transporter (CHT) messenger RNA and protein in the macaque monkey central nervous system. Signals for CHT mRNA and protein are localized in neurons in the motor nuclei of the cranial nerves (somatic motor and parasympathetic preganglionic), laterodorsal tegmental nucleus, parabrachial nucleus, pedunculopontine tegmental nucleus, globus pallidus, neostriatum, nucleus accumbens, nucleus of the diagonal band of Broca, nucleus basalis of Meynert, medial septal nucleus, and olfactory tubercle. Parasympathetic preganglionic neurons exhibited significantly lower density of CHT mRNA and protein than other positive neurons. In the spinal cord, motor neurons in the ventral horn showed both signals for CHT mRNA and protein. Putative gamma-motor neurons always showed strong immunoreactivity for CHT, while putative alpha-motor neurons showed … More wide variety of immunoreactivity intensities. Of putative alpha-motor neurons, neurons innervating principal dorsal muscles demonstrated higher content of CHT than neurons innervating limb muscles.2.Ultrastructural localization at the synapseTo determine whether CHT is located at the presynaptic membrane or at the membrane of synaptic vesicles, we analyzed the localization of CHT at the monkey neuromuscular junction using post-embedding immunocytochemistry with electronmicroscopy. Although immunoreactivity was weaker than reported in the rat, most of the immunoreactivity for CHT was located inside the nerve terminal rather than on the presynaptic membrane. The findings are compatible with the CHT localization on the vesicular membrane reported in the rat, but for the exact localization at the vesicular membrane, further study will be necessary to determine the optimal condition that preserve both the immunoreactivity for CHT and the structure of vesicular membrane. These electron microscopic studies were performed in collaboration with Professor Seiji Matsuda at Ehime University. Less