The mechanism of regeneration and repair in neurodegenaration animal model

已证实成年脑神经元具有再生能力。然而，确切的机制仍不清楚。首先，神经干细胞在多种神经营养因子和诱导因子的作用下增殖分化为神经胶质细胞和神经元，并逐渐成熟。这些是脊髓损伤，缺血性神经元死亡和癫痫。从损伤的早期，神经元干细胞增殖。这些细胞可以分化为神经胶质细胞和神经元细胞，因此这些细胞被称为多能祖细胞。为了阐明这些过程，我们研究了损伤后神经元祖细胞标志物巢蛋白（nestin）的表达情况，根据各细胞标志蛋白的表达情况，首先检测星形胶质细胞、少突胶质细胞和小胶质细胞的再生和活化。即使在小胶质细胞活化后，神经元细胞也开始再生。在脊髓挫伤模型中，我们检测到放射状胶质细胞的再生。这些细胞主要在胚胎阶段观察到，但在出生后下调。然而，我们在损伤后检测到这些细胞，并且放射状胶质细胞可以产生神经元和星形胶质细胞。这一过程的确切规则将予以澄清。

It has been clarified that neuron in adult brain can regenerate. However, the precise mechanism still unclear. First the neural stem cells proliferate and differenciate to gial cells and neuron by the several neurotrophic factors and inducer factors to matures cells.We have been studied about several neurodegeneration models. These are spinal cord injury, ischemic neuronal death, and epilepsy.From the early period of injury, neuronal stem cells proliferate. These cells can differentiate to glia and neuron, thus these are called as multipotent projenitor cells. In order to clarify these process, we studied nestin, neuronal projenitor cell marker, expression after the insult.According to the expression of each cells marker protein, we first detected the regeneration and activation of astrocytes, oligodendrocyte, and finally microglia. Even after microglial activation, neuronal cells begin to regenerate.In spinal cord contusion injury models we have detected the regeneration of radial glial cells. These cells mainly are observed in the embryonic stages, but are downregulated after birth. However, we detected these cells after injury, and neuron and astroglia can be produced from radial glia. The precise regulation of this process will be clarified.