Characterization of humic substances aimed at using for the removal of pollutants

腐殖质的亲水性与重金属的络合作用有关，而疏水性与多环芳烃的吸附作用有关。为了根据腐植酸的亲水性和疏水性对其进行表征，采用反相高效液相色谱法对腐植酸和黄腐酸进行了分析。采用反相高效液相色谱-荧光检测法测定了多环芳烃与重金属的络合作用和多环芳烃的吸附作用。此外，还研究了腐殖质对植物吸收重金属的影响。1.采用C18聚合物柱进行pH梯度洗脱和乙腈浓度梯度洗脱，根据腐植酸的亲水性和疏水性将腐植酸分成3个组分。2.采用聚合物整体柱提高分离度，将腐植酸和富里酸分离出5个…组分更多的比赛(I-V)。组分I是最亲水的，而组分V是最疏水的。黄腐酸以组分I为主，腐殖酸的组成随腐殖化程度的不同而不同。根据各组分的峰面积进行主成分分析，根据组分V的峰面积区分胡敏酸和黄腐酸，根据组分II-IV的峰面积区分A型胡敏酸和其他类型的胡敏酸。3.从荧光猝灭的角度估计，胡敏酸与重金属络合的组分是胡敏酸的组分I和II，而黄腐酸只有组分I。另一方面，根据稀土与腐殖质反应时荧光强度的增加，胡敏酸的吸附组分被认为是吸附多环芳烃的所有组分，富里酸的组分II到IV被认为是负责吸附多环芳烃的组分。4.堆肥腐殖质的应用增加了土壤中铜的溶解度，从而增加了印度芥菜对铜的吸收。较少

The hydrophilic property of humic substances is related to the complexation with heavy metals, whereas the hydrophobic property with the adsorption of polycyclic aromatic hydrocarbons (PAHs). To characterize humic substances based on their hydrophilicity and hydrophobicity, reverse-phased high performance liquid chromatography (RP-HPLC) was applied to humic and fulvic acids. The fractions responsible for the complexation with heavy metals and for the adsorption of PAHs were estimated by RP-HPLC with fluorescence detection. Furthermore, the effects of humic substances on the absorption of heavy metals by plant were investigated.1.When pH gradient elution and acetonitrile concentration gradient elution were applied to RP-HPLC using a C18 polymer column, humic acids were separated into three fractions according to their hydorphilicity and hydrophobicity.2.When used a polymer monolith column was used for RP-HPLC to increase resolving power, humic and fulvic acids were separated into five f … More ractions (I-V). The fraction I was most hydrophilic, whereas the fraction V most hydrophobic. Fulvic acids were characterized by a dominance of fraction I. For humic acids, however, the composition varied with the degree of humification. The principal component analysis using the peak area of each fraction showed that humic acid and fulvic acid were discriminated by the peak area of fraction V and the A type humic acids were discriminated from the other types of humic acids based on the peak areas of fractions II-IV.3.It was estimated from the quenching of fluorescence that the fractions responsible for the complexation with heavy metals were the fractions I and II for humic acids and only the fraction I for fulvic acids. On the other hand, the fractions responsible for the adsorption of PAHs were considered to be the all the fractions for humic acids and the fractions II to IV for fulvic acids based on the increase in the fluorescence when pyrene was react with the humic substances.4.The application of compost-derived humic substances increased the solubility of Cu in soil, and thus increased the absorption of Cu by Indian mustard (Brassica juncea). Less