Studies on the reduction of environmentall road in the waste water from rotifer culture by the introduction of Chemostat System

目前从法律的角度来看，孵化场的沃茨已经失控。但从“生态系统共存”的角度看，在不久的将来，应该实行“零排放”。在本项目中，我们通过对“从食物供给到轮虫的氮（N）转化效率”、“物质流”和“轮虫质量”的精密实验，与典型的普通生产系统--分批培养系统进行比较，讨论了“最新发明--恒化器-轮虫生产系统“的优点。增加游离氨的浓度会影响L型和S型轮虫的食物转化效率，导致水体中未同化食物的爆发。在恒化器系统中，考察了生产效率（轮虫N除以同化食物N）。L型轮虫的最大值为40%，高于S型轮虫的30%。总的来说，Chemostat系统的氨排放量低于分批培养系统，小于分批培养系统的四分之一。分批培养系统的细胞数远低于恒化器系统的细胞数，这可能是由于分批培养系统中的环境条件随着时间的推移而逐渐恶化。

Waste waters from hatcheries are out of control in the eye of the law at present. "Zero-emission" should be imposed, however, from the view point of "Exist together Ecosystems" in the near future. In this program, we discussed advantages of "Chemostat-rotifer-production-system, the newest invention" after precision experiments on "nitrogen (N) conversion efficiency from food provision to rotifer", "material flow" and "rotifer quality" in comparison with Batch-culture-system, the typical ordinary production system.In Batch-culture-system, increasing unionized-ammonia affected and lowered the food conversion efficiency in both L and S type rotifers, which induced an outbreak of unassimilated food in water. As a result, the concentration of organic substances was high compared by the amount of harvested rotifers.In Chemostat system, production efficiency (rotifer N divided by assimilated food N) was examined. The maximum value in L-type rotifer was 40% that was higher than the 30% in S-type rotifer. Collectively, ammonia emission from Chemostat system was lower than that from Batch-culture-system, and it was less than quarter of Batch-culture-system.As an index of the activity of harvested rotifers, carbon and nitrogen contents were evaluated. Those from Batch-culture-system were far less than the ones from Chemostat system, which may because of the worsening environmental conditions in Batch-culture as time elapsed.