Study on the seed production of agarophyte using tissue culture and its system development

研究了木脂植物组织培养的适宜选种和培养条件。适宜的培养条件为：20℃，60 ~ 100μmol/m^2 /s。在此条件下，植物生长调节剂浓度分别为：光丝生长用NAA 0.1 ~ 1μg/ml，盘状生长用NAA或4D 0.1 ~ 1μg/ml，盘状植物生长用ZEA 0.1μg/ml。另一方面，在此条件下，该树种长丝的适宜条件为：20℃，60 ~ 100μmol/m^2/s和。BAP 0.1 ~ 10μg/ml。将在此条件下生长的细丝转移到10℃、10 ~ 20μmol/m^2/s和。NAA浓度为0.1 ~ 10μg/ml，发育缓慢。但在20℃、60 ~ 100μmol/m^2/s和60 μmol/m^2/s的条件下，再生效果较好。BAP 0.1 ~ 10μg/ml。在20℃、40 ~ 60μmol/m^2/s的处理条件下，从花丝中萌发出盘状植株和选苗。通过这些结果，明确了江蓠适宜的培养条件，为江蓠的菌种培养和发展提供了可能。

Suitable species selection and culture condition of agarophytes using tissue culture were investigated. The most suitable species for this purpose is Gracilaria subpectinata Suitable culture conditions for this species are follows : 20℃, 60-100μmol/m^2 /s. The concentration of plant growth regulators under these conditions are follows : 0.1〜1μg/ml in NAA for filament growth, 0.1〜1μg/ml in NAA or 2, 4D for disc growth, and 0.1μg/ml in ZEA for elect plant growth from the disc.On the other hand, suitable condition for the filaments growth of this species under such condition are follows : 20℃, 60-100μmol/m^2/s and. 0.1-10μg/ml in BAP. When the filaments grown under this condition was transferred to the conditions of 10℃, 10-20μmol/m^2/s and. 0.1〜10μg/ml in NAA, they showed slow development. However, they showed good re-growth when they transferred to the conditions of 20℃, 60-100μmol/m^2/s and. 0.1〜10μg/ml in BAP. Furthermore discs and elect plants came up from the filaments under the conditions of 20℃, 40-60μmol/m^2/s.From these results it is cleared to find the suitable culture condition of Gracilaria subpectinata, which suggests they will be applied for its muss culture and a possibility for the development of culture.