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The development of evaluation method of peroxidation product and antioxidant of the food by the photon measurement.

The development of evaluation method of peroxidation product and antioxidant of the food by the photon measurement.
光子测量评价食品过氧化产物和抗氧化剂方法的开发
批准号:
15580219
负责人:
SAITO Takahiro
金额:
$2.05万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2004

项目摘要

项目成果

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中文摘要
翻译
活性氧和自由基的生成是超氧物,但由于这些生成过程有许多奢侈的种类和问号,因此被描述为评价有困难。因此,本文以XYZ活性氧清除体系为研究对象,构建了这类活性氧清除剂的评价技术。在XYZ体系中,当活性氧物种(X)与催化物种(Y)、受体(Z)同时共存时,光子释放(XYZ-发射)。实验结果表明:用三种标准试剂(X:2%H_2O_2a,Y:sat. C_5H_5COOH(OH)_3在10%CH_3CHO中的溶液,Z:饱和. KHCO_3的10%CH_3CHO溶液)中,XYZ-发射急剧增加,10-30秒后达到峰值,约为10^7cps。之后,它急剧下降,但XYZ排放强烈。此外,我们分析了这些XYZ发射,波长一直有600-800 nm的光谱,最大光谱为678 nm。在氧气和氮气、空气条件下,峰值XYZ-排放得到识别,但在XYZ-排放之后,响应于氧气量的增长。XYZ发射峰与标准X试剂的基数有相关性(相关系数:0.99)。适用于积分峰XYZ-发射的活性氧物种的评价方法。以水稻为参比材料,在不依赖大气的情况下,峰值XYZ发射后得到了识别和局部极小值。并利用该评价方法对水稻的XYZ发射峰与水稻体内元素含量的关系进行了检验。水稻XYZ排放峰值与脂肪酸指数相关,与输入量相关。脂肪酸指数是活性氧的产物,该测定方法能够对活性氧进行评价。这些结果证明了使用新的弱发射的活性氧物种的评价技术的适当性。
英文摘要
Agent was hyperoxide out of active oxygen and free radical, but was described as evaluation have difficult, because these generation process have many extravagant kind and question mark. So this paper focus on the XYZ active oxygen scavenging system for constructing of evaluation technique of these agent. This is premised on XYZ system : photon release (XYZ-emission) when active oxygen species (X) and catalytic species (Y), receptors (Z) coexist at the same moment. The following results were obtained : In which case mingle with three standard reagents (X:2% H_2O_2a,Y:sat. C_5H_5COOH(OH)_3 in 10% CH_3CHO,Z:sat. KHCO_3 in 10% CH_3CHO), XYZ-emission increased precipitously, and reach a peak of about 10^7 cps after 10-30 second. Afterward, it decreased precipitously, but XYZ-emission carried over mightily. Additionally, we analyzed these XYZ-emission, wavelength had spectrum of 600-800 nm all the time and maximum spectrum of 678 nm. Peak XYZ-emission get recognition in oxygen and nitrogen gas, air condition, but after XYZ-emission growth in answer to amount of oxygen gas. Peak XYZ-emission had a correlation with cardinal number of standard X reagent (coefficient of correlation : 0.99). Evaluation method of active oxygen species suited to integration peak XYZ-emission. Peak XYZ-emission from rice as reference material got recognition and local minimal value after peak XYZ-emission without recourse to atmosphere. And so we checked up relationship peak XYZ-emission and inside element in rice using this evaluation method. Peak XYZ-emission from rice had a correlation with fat acidity index and peak XYZ-emission had a correlation with input. Fat acidity index was product from active oxygen species, and this measurement method was able to evaluation of active oxygen species. These results demonstrated the adequacy of evaluation technique of active oxygen species using new weak emission.
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  • 项目类别:
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  • 资助金额:
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  • 财政年份:
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