Organogenesis of pancreas in PDX-1 promoter/YFP transgenic mouse

胰腺十二指肠同源盒-1(PDX-1)是胰腺器官发生的关键分子。为了进一步研究其在胰腺器官发生中的作用，我们建立了PDX-1启动子/YFP转基因小鼠。我们克隆了大鼠PDX-1启动子区域(-6.2kb)，并将其插入到pEYFP的多克隆位点。转基因建立了6个品系的小鼠。转基因动物生长发育正常，血糖水平也在正常范围。形态上，胰岛较大，胰岛β细胞数量增多。然而，胰升糖素阳性细胞在胰管附近形成簇状分布，胰管也是增生的。局灶性的淋巴管周围可见淋巴细胞浸润。需要详细分析转基因顺式元件在胰腺这些表型中的作用。

Pancreatic duodenal homeobox-1 (PDX-1) is the key molecule in organogenesis of the pancreas. To further investigate its roles in organogenesis of pancreas, we developed PDX-1 promoter/YFP transgenic mouse. We cloned rat PDX-1 promoter region (-6.2 Kb) and then inserted into multiple cloning site of pEYFP. Six lines of mouse were transgened and established. Development and growth of the transgenic animal were normal and blood glucose levels of those were also normal range. Morphologically, islets of those were relatively large, and number of beta cells was increased. However, the glucagons-positive cells formed a cluster and localized near the pancreatic duct, which were also hyperplastic. Focally, lymphocyte infiltration around the ducts was observed. It will be needed to analyze detail the role of the transgened cis element in those phenotypes in pancreas.