Genome-wide Association Study of Psoriasis Using Polymorphic Microsatellite Markers

近年来，对牛皮癣的全基因组研究取得了显著进展。这些研究已经在几条染色体上发现了许多银屑病易感基因。然而，银屑病的易感基因尚未确定。在这项研究中，我们系统地进行了银屑病的关联分析，以确定所有银屑病的易感基因。本研究的研究对象为无血缘关系的日本寻常型银屑病患者。第一、二、三组共收集独立样本125个。同样，将125名独立的、无血缘关系的健康个体作为对照，将其合并为第1、2、3组。我们用第一套混合DNA进行了第一次筛查。之后，使用第二和第三组进行第二和第三次筛查，以避免假阳性关联。结果，可能的牛皮癣候选基因被缩小到不到40个区域。

Genome-wide studies of psoriasis has made remarkable progress recently. These studies have identified many psoriasis susceptibility loci on several chromosomes. However, psoriasis susceptibility genes have not identified. In this study, we have systematically performed association analysis of psoriasis to define all psoriasis susceptibility loci. The subjects investigated in this study were unrelated Japanese patients with psoriasis vulgaris. Independent 125 samples were pooled for the 1st, 2nd and 3rd sets. Similarly, independent 125 unrelated healthy individuals as controls were pooled for the 1st, 2nd and 3rd sets. We used the 1st set pooled DNA for the 1st screening. After that, 2nd and 3rd sets were used for the 2nd and 3rd screening to avoid false positive associations. As the result, putative psoriasis candidate loci were narrowed down to less than 40 regions.