Ultrastructural enzymehistochemical and cell-biological analysis of the preterm dilivery -relationship between fetal cells and preterm delivery-

1.研究了足月妊娠人羊膜上皮细胞的形态。在化学固定的羊膜中，有两种不同程度的细胞质暗。而高尔基体和线粒体的数量和形态在细胞间无明显差异。酶组织化学检测到的酶的分布模式和强度在不同细胞之间也没有差异。冷冻替代固定消除了细胞间胞质黑暗的差异。从形态特征上看，人羊膜上皮细胞是由均一的细胞群组成。2.早产孕妇绒毛组织中有丰富的中性粒细胞。这些白细胞具有吞噬白细胞的形态特征。吞噬膜上出现髓过氧化物酶阳性的吞噬空泡。这些激活的白细胞正好聚集在绒毛膜基底膜的前面。我们的结论是，在早产的绒毛羊膜中，母血白细胞聚集在该组织的基底膜上，并吞噬了该膜上的各种细胞碎片。这种现象可能与早产的发生密切相关。3.我们在超微结构水平上建立了酸性磷酸酶和葡萄糖-6-磷酸脱氢酶组织化学双重染色。我们将这项技术应用于早产胎儿胎盘巨噬细胞。葡萄糖-6-磷酸脱氢酶染色阳性的巨噬细胞定义为活化的巨噬细胞。早产胎盘中活化的胎盘巨噬细胞数量明显多于足月分娩胎盘。结论胎儿胎盘巨噬细胞与早产的发生密切相关。

1.We characterized the morphology of the human amniotic epithelial cells in term pregnancies. In the chemically fixed amnion, there were two types of cells with different degree of cytoplasmic darkness. However, there were no differences in the number and the shape of Golgi apparatus and mitochondria between the cells. The distribution pattern and the intensity of the enzyme detected by the enzymehistochemistry did not differ between cells, either. Freeze-substitution fixation abolished the differences of the cytoplasmic darkness between cells. We concluded that human amniotic epithelial cells consisted of the homogeneous cell population as far as the morphological features were concerned.2.In the chorioamnion from women with preterm delivery there were abundant polymorphonuclear leukocytes. These leukocytes exhibited morphological features characteristic of phagocytosing leukocytes, ie. : appearance of phagocytotic vacuole with myeloperoxidase on the phagocytotic membrane. These activated leukocytes accumulated just in front of the basement membrane of the chorioamnion. We concluded that in the chorioamnion of the preterm delivery maternal leukocytes accumulated at the basement membrane of this tissue and they phagocytosed various cell debris in this membrane. The phenomenon observed here may have close association with the development of the preterm delivery.3.We developed dual staining for acid phosphatase and glucose-6-phosphate dehydrogenase histochemistry at an ultrastructural level. We applied this technique to the fetal placental macrophages in preterm delivery. The macrophages with positive staining for glucose-6-phosphate dehydrogenase were defined as activated macrophages. The number of activated fetal placental macrophages were significantly larger in the placenta with preterm delivery than that with normal term delivery. We concluded that fetal placental macrophages had close association with the initiation of the preterm delivery.