Arginine effect on the absorption and motor functions of the residual ileum after massive small intestinal resection

【目的】本研究采用适合研究精氨酸在体内重要性的短小肠模型，建立同时评估残回肠吸收和运动功能的实验体系。[方法]1)4周龄雄性SD大鼠，切除90%近端小肠，将剩余小肠在回盲瓣近端5cm处吻合。接受手术的大鼠和小肠正常的大鼠分别服用精氨酸或甘氨酸。按此方法共制备四组。2)采用遥测系统分析肠蠕动。为了记录残余回肠的运动功能，在腹腔内给药Nembutal后，用6-0尼龙将一个4 mm的力传感器（FT）缝合在离吻合口远2 cm的回肠薄而脆弱的肠壁上。缝合前给予0.1 mg/kg硫酸阿托品皮下注射。使用小型发射器（小鼠），用5-0丝固定在腹腔内腹壁上，自FT包埋后3天开始重复记录圆形肌肉的收缩情况。在近端小肠切除90%后3周和6周，每小时计算禁食前12小时和禁食后24小时的收缩波积分值。3)术后3周和6周，通过食道给药50 mg d -木糖，并利用10小时尿样计算尿排泄率，评估吸收功能。[结果]1)对于精氨酸，餐后每小时收缩波积分值小于禁食前；然而，对于甘氨酸，禁食前后没有差异。在禁食期间出现的大收缩运动的次数和持续时间被认为相当于迁移的肌电（或运动）复合体（MMC），与精氨酸的使用没有相关性。2)术后3、6周测定吸收功能。虽然精氨酸在术后3周和4周改善了吸收，但在术后6周没有进一步改善。【结论】精氨酸仅在术后3周内改善短小肠模型的吸收功能。因此，为了确定精氨酸对肠道运动的影响，需要进一步的研究。少

[Objective]The present study was conducted, using our short-small intestine model that is suitable for investigating the importance of arginine in vivo, in order to establish an experiment system that simultaneously assesses the absorption and motor functions of the residual ileum.[Methods]1)Using 4-week-old male SD rats, 90% of the proximal small intestine was excised and the remaining intestine was anastomosed 5 cm proximal to the ileocecal valve. Either arginine or glycine was administered to rats that underwent this surgery and rats with a normal small intestine. A total of four groups were prepared in this manner.2)Intestinal motility was analyzed using the Telemeter System. In order to record the motor function of the residual ileum, a 4-mm force transducer (FT) was sutured to the thin and fragile wall of the ileum 2 cm distal to the anastomotic site using 6-0 nylon, after intraperitoneal administration of Nembutal. Before suturing the FT, 0.1 mg/kg of atropine sulfate was admini … More stered subcutaneously. Using a small transmitter (for mice), fixed to the abdominal wall inside the abdominal cavity using 5-0 silk, contraction of the circular muscle was recorded repeatedly from 3 days after the embedding of the FT. At 3 and 6 weeks after 90% proximal small intestinal resection, the integral values for contraction waves before and after 12 or 24 hours of fasting were calculated every hour.3)Absorption function was assessed at 3 and 6 weeks after surgery by esophageally administering 50 mg of D-xylose and calculating the absorption rate based on urinary excretion using a 10-hour urine sample.[Results]1)For arginine, the integral values for hourly contraction waves after a meal was smaller than that before fasting ; however, for glycine, no difference between before and after fasting was observed. The number and duration of large contractile motions, that appeared during fasting and were thought to be equivalent to migrating myoelectric (or motor) complexes (MMC), showed no correlation to arginine administration.2)Absorption function was measured at 3 and 6 weeks after surgery. While arginine administration improved absorption at 3 and 4 weeks after surgery, no further improvement was seen at 6 weeks after surgery.[Conclusions]Arginine improved the absorption function of the short-small intestine model for only three weeks after surgery. Therefore, further investigations are required in order to ascertain the effects of arginine on intestinal motility. Less