Study on alteration of expression of genes and proteins related to development, progression and characteristic morphogenesis of salivary gland tumors

本研究以涎腺肿瘤组织和细胞系为材料，检测了涎腺肿瘤中基因和蛋白表达的变化，为涎腺肿瘤的诊断、预测和治疗积累基础资料.正常涎腺上皮细胞强表达的细胞粘附分子如E-钙粘素、β-连环素、CD 44 v9，细胞周期调控因子如p53、p27、cyclin D1、Aurora A/B及表皮生长因子受体在涎腺肿瘤尤其是恶性肿瘤中的表达发生改变。在腺样囊性癌中，Skp 2可能通过下调p27而在其发生、发展过程中发挥重要作用。在此基础上，我们将提出一个新的低度恶性多形性腺瘤的实体与p53基因突变和p27蛋白下调.萨基和periostin与口腔鳞状细胞癌的发生和/或进展相关，可能与涎腺肿瘤的发生和/或进展无关.在嗜酸细胞化生导致涎腺肿瘤诊断困难的肿瘤中，评估包括腺上皮和肌上皮标志物在内的鉴别标志物对诊断的建立至关重要.通过转染hTERT基因建立了表达腺上皮和肌上皮标志物的永生化多形性腺瘤细胞。这些细胞有助于分析多形性腺瘤的特征性形态发生。

To accumulate the basic data useful to diagnosis, prediction and treatment of salivary gland tumors, we examined the alteration of gene and protein expression in various salivary gland tumors using tumor tissues and cell lines.1. The expression of cell adhesion molecules such as E-cadherin, β-catenin and CD44v9 which are strongly expressed in epithelium of normal salivary gland, cell cycle regulatory factors such as p53, p27, cyclin D1 and Aurora A/B and epidermal growth factor receptor altered in salivary gland tumors, especially malignant tumors. In adenoid cystic carcinoma, Skp2 may play an important role in development and pregression through the down-regulation of p27. On the basis of these results, we will propose a new entity of low-grade malignant pleomorphic adenoma with mutation of p53 gene and down-regulation of p27 protein.2. SAKI and periostin related to the development and/or progression of oral squamous cell carcinoma may be not associated with those of salivary gland tumors.3. In the tumors with oncocytic metaplasia which causes the diagnostic difficulty in salivary gland tumors, the evaluation of differential markers including glandular epithelial and myoepitheial markers is important for the establishment of the diagnosis.4. The immortalized pleomorphic adenoma cells showing the expression of glandular epithelial and myoepithelial markers were established by transfection with hTERT gene. These cells are useful for the analysis of characteristic morphogenesis of pleomorphic adenoma.