Genetic diagnosis and therapy targeting the Wnt signaling pathway of oral cancer

探讨纤溶酶原激活物/纤溶酶系统在口腔鳞状细胞癌(SCC)细胞E-钙粘蛋白表达和功能中的作用。纤溶酶原处理SCC细胞后，细胞对钙离子的依赖聚集减少。鳞状细胞癌细胞在细胞聚集区表达E-钙粘蛋白。SCC细胞在细胞膜上表达E-钙粘附素，并在80 kDa处释放少量可溶性E-钙粘附素。在SCC细胞中加入纤溶酶原后，细胞膜上E-钙粘附素的数量减少，E-钙粘附素外膜粘附素的脱落增加。纤溶酶直接裂解鳞状细胞癌细胞上的E-钙粘附素，增强鳞癌细胞的运动能力。提示纤溶酶原激活剂/纤溶酶系统可能直接参与口腔鳞癌细胞中E-钙粘附素的蛋白降解过程，并可能通过下调E-钙粘附素介导的细胞间黏附而促进口腔鳞状细胞癌的进展。

The participation of plasminogen activator/plasmin system in the expression and function of E-cadherin was examined in oral squamous cell carcinoma (SCC) cells. Treatment of SCC cells with plasminogen reduced the Ca2+ -dependent cell aggregation. SCC cells expressed E-cadherin at the cell aggregation. SCC cells expressed E-cadherin at the membrane, and released a small amount of soluble E-cadherin at 80 kDa in the culture medium. Addition of plasminogen to SCC cells led to a decrease in the amount of E-cadherin of the cell membrane and the enhancement of the shedding of E-cadherin Ectodomein. Plasmin, directly cleaved E-cadherin of SCC cells and enhanced the motility of SCC cells. these results suggested that plasminogen activator/plasmin system might directly mediate the proteolytic processing of E-cadherin in oral SCC cells and that might facilitate the progression of oral SCC by downreguration of E-cadherin-mediated cell-cell adhesion.