Prognostic prediction in lupus nephritis using CARP which is newly identified as a podocyte marker.

心脏锚定重复蛋白（Cardiac anklost-repeated protein，CARP）最初被鉴定为在心肌细胞中特异表达的蛋白质，但最近发现在一些肌肉疾病中表达上调，包括肌营养不良和肌病，提示CARP可能在某些病理条件下被诱导。我们发现CARP在新月体肾小球肾炎（GN）、糖尿病肾病（DN）和狼疮性肾炎（LN）的肾足细胞中有高水平表达，而在正常肾脏中不表达。有趣的是，在LN中，CARP的表达倾向于在有肾病综合征的病例中被诱导，而在没有肾病综合征的病例中较少，这表明CARP的表达与蛋白尿的严重程度相关。 ...更多信息 霉素。当将LPS和阿霉素注射到小鼠中时，在共表达足细胞标记物突触足蛋白的肾小球细胞中诱导CARP，而在注射PBS的小鼠中不诱导CARP。因此，这是强烈建议，CARP确实是诱导足细胞在足细胞损伤的反应。大鼠肾小球壁上皮细胞（PEC）在LPS和血管紧张素II处理后发生形态学改变，并出现膜皱褶。有趣的是，鬼笔环肽染色检测到F-actin在这些膜皱褶区域的积累。当我们用血管紧张素受体阻断剂（ARB）处理时，形态和分布的变化被部分阻断。另一方面，我们对例肾小球肾炎患者尿液中的CARP阳性细胞进行了化学分析。我们可以检测到少量的CARP阳性细胞。虽然我们也试图确定一种单克隆抗体对CARP，我们还没有确定它you.In结论，这表明，免疫组化评价CARP活检标本或尿液LN患者可能是有用的疾病的严重程度的诊断，并可能为LN患者的预后预测。少

Cardiac ankyrin-repeated protein (CARP) was originally identified as a protein specifically expressed in cardiomyocytes, but has recently been found to be up-regulated in some muscle diseases including muscular dystrophy and myopathy, suggesting that CARP may be induced in some pathological conditions.In this study, we immunohistochemically analyzed renal biopsy samples from patients with glomerular diseases and two individuals with normal kidney. We found that CARP was expressed in renal podocytes at a high level in crescentic glomerulonephritis (GN), diabetic nephropathy (DN), and lupus nephritis (LN), although it was not expressed in normal kidney. Interestingly, in LN, CARP expression tended to be induced in cases exhibiting nephrotic syndrome, but less so in cases without nephrotic syndrome, suggesting that CARP expression is correlated with the severity of proteinuria.Then we analyzed the expression of CARP in podocytes of Balb/c mice that had been administrated with LPS or adria … More mycin. When LPS and adriamycin were injected to mice, CARP was induced in glomerular cells that co-express synaptopodin, a marker for podocyte, whereas CARP was not induced in mice injected with PBS. Thus, it is strongly suggested that CARP is really induced in podocytes in response to podocyte-injury. Rat glomerular parietal epithelial cell (PEC) underwent morphological changes and exhibited membrane ruffling after treatment with LPS and angiotensin II. Interestingly, phalloidin staining detected accumulation of F-actin in these areas of membrane ruffling When we treated with anagiotensin receptor blocker (ARB), morphological and distributional changes were partially blocked. On the other hand, we immunohistochemically analyzed the CARP-positive cells in urine frojm patients of GN. We could detect a small number of CARP-positive cells. Although we also tried to identify a monoclonal antibody against CARP, we have not identified it yet.In conclusion, it is suggested that immunohistochemical evaluation of CARP in biopsy samples or urine from patients with LN may be useful for diagnosis of disease severity, and probably for prediction of outcome in LN-patients. Less