Effects of perinatal combined exposure to organic pollutants on rat offspring

研究了三丁基锡（TBT）、1，4-二氯苯（DCB）和p，p '-DDE（DDE）联合暴露对妊娠期和哺乳期母鼠子代的影响。将母鼠分为对照组、TBT或DCB组、TBT或DCB+DDE组。对照组喂饲正常大鼠饲料，CE-2、DCB组喂饲含25 ppm（2 mg/kg）TBT或DCB的CE-2，TBT或DCB+DDE组喂饲含25 ppm TBT或DCB和125 ppm（10 mg/kg）DDE的CE-2。母鼠从妊娠第1天至产后第21天饲喂制备好的饲料42天。在出生后第2 - 4天，对雄性和雌性大鼠的后代进行扑杀，以避免由于喂养状态导致的生长差异。大鼠后代在断奶后饲喂CE-2。在接受治疗的母鼠中未观察到妊娠和生殖结局恶化。围产期联合暴露于三丁基锡化合物导致大鼠后代生长迟缓，而暴露于二氯苯则导致雌性后代胸腺保留。然而，这些措施被同时暴露于DDE所抵消。此外，在本实验中观察到DCB和DDE暴露的协同效应，并观察到卵巢重量减少，这可能导致生殖功能障碍。

Effects of perinatal combined exposure to tributyltin (TBT) or 1,4-dichlorobenzene (DCB), and p, p'-DDE (DDE) were investigated in rat offspring of dams ingesting TBT or DCB, and DDE during the gestational and lactational period. Dams were divided into three groups, respectively; namely control group, TBT or DCB group and TBT or DCB+DDE group. Control group rats were fed normal rat chow, CE-2,DCB group rats were given CE-2 containing 25ppm (2 mg/kg) TBT or DCB, and TBT or DCB+DDE group rats were administered CE-2 containing 25ppm TBT or DCB and 125ppm (10 mg/kg) DDE. Dams were fed prepared feed for 42 days from gestational day 1 to postpartum day 21. Rat offspring were culled on postnatal day 2 to 4 males and females each, in order to avoid differences of growth due to feeding states. Rat offspring were fed CE-2 after weaning. No deteriorated pregnancies and reproductive outcomes were observed in treated dams. Perinatal combined exposure to TBT produced growth retardation in rat offspring and that to DCB induced thymus preservation in female offspring. However, these actions were counterbalanced by concurrent exposure to DDE. Furthermore, synergistic effects of exposure to DCB and DDE were observed and the reduction of the ovary weight was seen in this experiment, which might lead to reproductive dysfunction.