The effects of anesthetics on aquaporin-5

利用非洲爪哇表达系统检测麻醉剂对水通道蛋白-5(AQP5)功能的影响。显微注射体外转录的AQP5基因的非洲爪哇卵母细胞表现出上述体积的增加；这可被已知的水通道抑制剂氯化汞可逆地抑制。由渗透效应引起的体积变化率在连接到面积分析仪的照相机上成像。序列图像以1秒的间隔存储在计算机中。比较了戊巴比妥钠(50-1000um)、异丙酚(1-200um)、氯胺酮(1-100um)、咪达唑仑(1-200um)、七氟醚(1 Mm)、异氟醚(1 Mm)、利多卡因(1um)、苯佐卡因(1um)和QX-314(1um)等不同麻醉药的作用。戊巴比妥和异丙酚可抑制体积变化率，而氯胺酮和咪达唑仑对此无影响。而七氟醚和异氟醚增加了AQP5的体积变化率。利多卡因、苯佐卡因(永久不带电)和QX-314(永久带电)对容量变化率无影响。这些结果提示，某些麻醉药可能确实影响了AQP5的功能。

We examined the effects of anesthetic agent for the function of aquaporin-5 (AQP5) by Xenopus expression system. Oocytes from Xenopus Laevis microinjected with in vitro-transcripted AQP5 mRNA exhibited increased these volume; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. The rate of volume change due to osmotic effects was imaged on the camera connected to an area analyzer. Serial images were stored at 1-sec intervals in a computer. We compared actions of different anesthetic drugs, such as pentobarbital (50-1000 uM), propofol(1-200 uM), ketamine (1-100 uM), midazolam (1-200 uM), sevoflurane (1 mM) and isoflurane (1 mM), lidocaine (1 uM), benzocaine (1 uM) and QX-314 (1 uM). The ratio of volume change was inhibited by pentobarbital and propofol while ketamine and midazolam had no effect. On the other hand, sevoflurane and isoflurane increased the ratio of volume change by AQP5. Lidocaine, benzocaine (permanently uncharged) and QX-314 (permanently charged) had no effects for the ratio of volume change. These results suggest that some anesthetics may indeed affect the function of AQP5.