Laser-induced transient absorption and photoacoustic spectrometer

功能材料的光物理研究对于在(超)分子水平上深入理解激发态性质起着基础性作用。对于正在进行的和未来的地方、国家和国际层面的项目，需要最新的光谱和时间分辨表征方法；因此，激光诱导瞬时吸收光谱需要与光声光谱相结合。这将构成一种目前在这种形式下无法获得的独特组合，同时使非发光状态的检测成为可能。在可变温度下测量具有光谱和时间分辨率的溶液、均质基质、纤维、纳米或微粒子中的单体或聚集分子的暗激发态的性质是一个特别的挑战。这也适用于需要用光谱和时间分辨率来评估光敏剂、荧光或磷光探针在培养介质、细菌、细胞器和细胞中的激发态性质的情况。特别是，需要监测与基质、细菌和真核生物(宿主细胞或原生动物寄生虫)成分的具体相互作用。这包括导致具有可变寿命的非发光状态或自由基离子的光致电荷或能量转移过程。这些挑战将通过所要求的仪器来克服，这将使这种物种的检测成为可能，除了量化它们的能量含量之外。这种瞬时吸收和光声光谱的结合将促进分子生物成像和光疗策略的新标记技术的发展，同时带来光电子学的新概念。

The photophysical investigation of functional materials plays a fundamental role towards an in-depth understanding of excited state properties at (supra)molecular level. For ongoing and future projects at local, national, and international level, state of the art spectrally and temporally resolved characterization methods are required; hence, laser-induced transient absorption spectroscopy needs to be combined with photoacoustic spectroscopy. This will constitute a unique combination that is presently not available in this form, while enabling the detection of non-luminescent states.Measuring the properties of dark excited states from monomeric or aggregated molecules in solutions, homogeneous matrices, fibres, nano- or microparticles with spectral and temporal resolution at variable temperatures poses a particular challenge. This also applies when the excited state properties of photosensitizers, fluorescent or phosphorescent probes need to be assessed with spectral and temporal resolution in culture media, bacteria, organelles and cells. In particular, the specific interactions with matrixial, bacterial and eukaryotic (hosts cells or protozoic parasites) components need to be monitored. This includes photoinduced charge- or energy-transfer processes leading to non-luminescent states with variable lifetimes or to radical ions. These challenges will be overcome with the requested instrumentation, which will enable the detection of such species, besides the quantification of their energy content. This combination of transient absorption and photoacoustic spectroscopies will boost the development of new labelling techniques for molecular bioimaging and phototherapeutic strategies while leading to new concepts for optoelectronics.