Highly functional utilization of surplus renewable resources through microbial regio-specific hydroxylation

研究了丁香酚、异丁香酚、松香等再生资源的微生物转化。本研究分离出一株降解丁香酚的荧光假单胞菌E118，并尝试建立从丁香酚和丁香油中提取有用化学物质阿魏酸的微生物生产工艺。我们还分离出一种降解异丁香酚的细菌，恶臭假单胞菌，并试图对异丁香酚的微生物降解的轮廓进行阐明。P.putida 158静息细胞通过香兰素快速将异丁香酚转化为香草酸，转化率达98%。估计了催化异丁香酚双键直接裂解的酶的出现。我们还分离到松香转化微生物，Mucor circinelloides，显示出较高的羟基化活性。积累了大量的2 -羟基枞酸，转化率高。通过本文的研究，获得了丁香酚、异丁香酚和松香的微生物代谢的一些重要结果。基于有价值的信息和知识，我们打算建立从可再生资源丁香酚、异丁香酚、丁香油和松香中提取有用化合物的新工艺。

The microbial conversion of renewable resources such as eugenol, isoeugenol, and rosin was studied. We isolated an eugenol-degrading bacterium, Pseudomonas fluorescens E118, and we attempted to establish the microbial production process of useful chemicals, ferulic acid, a useful antioxidant, from eugenol and clove oil. We also isolated an isoeugenol-degrading bacterium, Pseudomonas putida, and attempted to throw the light on the outline of the microbial degradation of isoeugenol. The resting cells of P.putida 158 rapidly converted isoeugenol into vanillic acid via vanillin with a conversion yield of 98%. The occurrence of the enzymes to catalyze the direct cleavage of double bond of isoeugenol was estimated. We also isolated rosin-converting microorganism, Mucor circinelloides which showed the high hydroxylation activity. The high amount of 2a-hydroxyabietic acid was accumulated with a high conversion yield. Some significant results regarding the microbial metabolisms of eugenol, isoeugenol and rosin were obtained through the present thesis. Based on the valuable information and knowledge, we intended to establish new production process of useful compounds from renewable resource, eugenol, isoeugenol, clove oil and rosin.