Morphological changes of endothelial cells and pharmaceutical actions of drugs and poisons using the cells.

药物和毒药的毒性表现为人体的致死剂量。本研究以人内皮细胞（HUVEC）为研究对象，探讨了致死剂量与细胞死亡剂量之间的关系。用药物和毒物混合培养液的形态学变化判断HUVEC的死亡。使用甲基苯丙胺（MA）、可卡因、苯巴比妥（PB）、百草枯（PQ）的细胞死亡剂量分别为5、5、5和0.1 mM。其他57种药物和毒物用同样的方法进行筛选。毒性最大的是亚砷酸钠（0.01mM），毒性最小的是甲醇（2M）。根据文献，MA、可卡因、PB、PQ的人体致死剂量（含推定值）分别为1.3 ~ 20mg /kg、7 ~ 10mg /kg、14 ~ 200mg /kg、14 ~ 40mg/kg。为了比较致死剂量和细胞死亡剂量的值，单位取mg/L和mg/kg。MA、可卡因、PB和PQ的浓度分别为0.007 ~ 0.1、0.002 ~ 0.003、0.06 ~ 0.9和0.05 ~ 0.16 mm。在MA中，可卡因和PB的人致死剂量与HUVEC细胞死亡相比为1/6 ~ 1/700。在PQ中，人的剂量与HUVEC细胞死亡水平相同。用同样的方法，绘制了49种物质的人体致死剂量与HUVEC细胞死亡之间的关系。绘制关系线时，相关系数(r)为0.310，说明相关性较差。综上所述，在所检测的61种药物和毒物中，HUVEC的形态学变化与人的致死剂量关系不大。致死剂量与人内皮细胞死亡之间的关系有待进一步研究。

Toxicity of drugs and poisons shows as lethal dose in human. In this study, relationship between the lethal dose and dose of the cell death was examined using human endothelial cells(HUVEC). Death of HUVEC judged with morphological changes in culture fluids mixed with drugs and poisons. Doses of the cell death using methamphetamine(MA), cocaine, phenobarbital(PB), paraquat(PQ) showed 5,5,5 and 0.1 mM, respectively. The other 57 drugs and poisons were screened with the same method. The most poisonous substance was arsenite sodium (0.01mM) and the least poisonous one was methanol(2M). By the references, lethal doses of human(including presumption values) in MA, cocaine, PB and PQ were 1.3-20,7-10,14-200 and 14-40mg/kg, respectively. To compare the values between lethal dose and dose of cell death, the unit made mg/L of mg/kg. The concentrations of MA, cocaine, PB and PQ were 0.007-0.1,0.002-0.003,0.06-0.9 and 0.05-0.16mM, respectively. In MA, cocaine and PB lethal doses of human showed 1/6 to 1/700 comparing to cell death of HUVEC. In PQ, however, the dose of human was same level as cell death of HUVEC. In the same manner, 49 substances were plotted between lethal dose of human and the cell death of HUVEC. When the relation line was made, the correlation coefficient(r) was 0.310, indicating poor relationship. In conclusion, morphological changes of HUVEC showed poor relationship to lethal dose of human in total 61 drugs and poisons examined. Further examination needs to determine the relationship between lethal dose and endothelial cell death in human.