Anti-neural antibodies induced by brain injury and analysis of their antigen

本研究的目的是检测和分析实验性脑损伤诱导的抗神经元抗体，以阐明特异性抗神经元抗体在某些神经系统疾病中的意义。1.选用6月龄、2岁龄雌性Sprague-Dawley大鼠，用解剖刀造成双侧残端创伤，分别于术前和术后4周采集血清。以大鼠大脑、脑干和肝脏为抗原，用Western blot法检测血清中是否含有新合成的抗神经抗体。2.收集3例脑梗死患者发病当天及1个月后的血清，其中3对无一对，均未检测到新合成的抗神经抗体。系统的灵敏度、来自大鼠的有限量的血液被认为有可能无法检测抗体。应检查是否有炎性脑损伤引起抗神经抗体。

Objects of our studies were detection and analysis of anti-neural antibodies induced by experimental brain injury, which may elucidate significance of the specific anti-neural antibodies in some neurological disease.1.Spraque-Dawley female rats (6 month, 2 year age) were used, Bilateral stub wounds were made with a dissecting knife, Sera from rats were collected before and 4 weeks after operation. Sera were examined if they contained newly synthesized anti-neural antibodies by the Western blot using rat cerebrum, brainstem and liver as antigens.2.Sera from 3 patients with cerebral infarction were collected at the day of onset and one month later, 3 pairs Non pair had that, None newly synthesized anti-neural antibody was detected in rat or human. Sensitivity of the system, limited amount of blood from rats are thought of the possibility to fail detection of antibody. Inflammatory insult to brain should be examined if it cause the anti-neural antibodies.