Cloning and characterization of a novel neuropeptide using TRH knockout mice.

据报道，TRH在大脑中具有多种神经生理学作用。为了深入了解这些影响的分子机制，我们试图克隆一个由TRH基因敲除小鼠和消减cDNA分析和微阵列系统的TRH调节的cDNA。分析了通过野生型和TRH^<-/->小脑之间的这些分析获得的超过100个克隆。其中，一个克隆，补充甲状腺激素的甲状腺功能正常的TRH^<-/->小脑中的mDP 1 mRNA水平与野生型相比显著降低。北方印迹分析显示，mDP 1 mRNA在下丘脑、纹状体、中脑、海马、脑桥、小脑和皮质等7个脑区均有表达。饥饿72 h后，下丘脑和小脑mDP 1 mRNA水平显著下降。这些结果表明，mDP 1可能是一种调节饱足行为的物质。

TRH has been reported to possess several neurophysiological actions in the brain. To gain insights into the molecular mechanisms underlying these effects, we attempted to clone a cDNA that was regulated by TRH using TRH knockout mice and subtractive cDNA analysis and a microarray system. Over 100 clones obtained by these analyses between the wild-type and TRH^<-/-> cerebellum were analyzed. Among them, a clone, mDP1 mRNA level in the euthyroid TRH^<-/-> cerebellum supplemented with thyroid hormone were significantly decreased compared with those in the wild-type. Northern blot analysis of seven brain regions including hypothalamus, striatum, midbrain hippocampus, pons, cerebellum, and cortex revealed that mDP1 mRNA were expressed significantly in the hypothalamus and cerebellum. Furthermore, starvation for 72 hours lead to a significant decrease in mDP1 mRNA level in the hypothalamus and cerebellum. These results suggested that mDP1 might be a substance for regulating satiety behavior.