Analysis of expression for HsAIRK and PLK family genes, that regulate chromosome segregation in mitosis, in tumors

Aurora家族在M期高度表达，并被认为调节着中心体功能、染色体分离和细胞质分裂。此外，PLK家族还密切参与有丝分裂过程中的纺锤体形成和染色体分离。本研究的目的是确定与正常结肠粘膜相比，PLK或Aurora家族在原发性结直肠癌标本中是否过表达，并评估其与其他激酶的关系，作为一种潜在的新肿瘤标志物。在本研究中，我们对78例原发性结直肠癌和15例正常结直肠癌标本进行了PLK1表达的免疫组织化学分析。此外，我们还检测了其他激酶，Aurora-A和Aurora-C与PLK1表达的关系。在15例正常结肠黏膜中，13例隐窝细胞PLK1呈弱阳性染色。57例（73.1%）结直肠癌患者PLK1表达升高，与pT、pN及Dukes分级有显著相关性。PLK1高表达和低表达病变值分别为54.7±10.3% （mean±SD）和45.9±11.9%。PLK1与Aurora-A有显著相关性，但与Aurora-A或aurora - c - 2相比，PLK1的染色更为弥漫性和广泛性。本研究对78例原发性结直肠癌、36例结直肠腺瘤和15例正常结直肠标本中HsAIRK1和HsAIRK3的表达进行了免疫组化分析。在53例（67.9%）结直肠癌患者中观察到HsAIRK1表达升高，在40例（51.3%）结直肠癌患者中观察到HsAIRK3表达升高。结直肠腺瘤中分别有11例（30.6%）和7例（19.4%）高表达HsAIRK1和HsAIRK3，介于结直肠癌和正常结直肠粘膜之间。有趣的是，在结直肠癌中，HsAIRK1过表达与p53积累显著相关。我们的研究结果表明，PLK1或Aurora A的过表达可能具有致病、预后和增殖的重要性，因此这些有丝分裂相关基因可能有潜力作为结直肠癌的新肿瘤标志物。少

Aurora family are highly expressed during M phase, and have been suggested to regulate centrosome function, chromosome segregation, and cytokinesis. In addition, PLK family is also intimately involved in spindle formation and chromosome segregation during mitosis. The purpose of this study was to determine whether PLK or Aurora family are overexpressed in primary colorectal cancer specimens as compared with normal colon mucosa and assess its relation to other kinases as a potential new tumor marker.1.PLKIn the present study, immunohistochemical analyses were performed of PLK1 expression in 78 primary colorectal cancers as well as 15 normal colorectal specimens. Furthermore, we examined the relationship between other kinases, Aurora-A and Aurora-C, and PLK1 expression. In normal colon mucosa, some crypt cells showed weak positive staining for PLK1 in 13 out of 15 cases. Elevated expression of PLK1 was observed in 57 (73.1%) of the colorectal cancers, statistically significant associatio … More ns being evident with pT, pN and the Dukes' classification. Values for lesions with high and low PLK1 expression were 54.7±10.3% (mean±SD) and 45.9±11.9%. PLK1 was significantly associated with Aurora-A, but PLK1 staining was more diffuse and extensive than for Aurora-A or Aurora-C.2.AuroraIn the present study, immunohistochemical analyses were performed of HsAIRK1 and HsAIRK3 expression in 78 primary colorectal cancers and 36 colorectal adenomas as well as 15 normal colorectal specimens. Elevated expression of HsAIRK1 was observed in 53 (67.9%) of the colorectal cancers, and of HsAIRK3 in 40 (51.3%). Furthermore, colorectal adenomas showed high expression of HsAIRK1 and HsAIRK3 in 11 (30.6%) and 7 (19.4%) cases, respectively, thus being intermediate between colorectal cancers and normal colorectal mucosa. Interestingly, HsAIRK1 overexpression was significantly associated with p53 accumulation in colorectal cancers.Our results suggest overexpression of PLK1 or Aurora A might be of pathogenic, prognostic and proliferative importance, so that these mitosis-related genes might have potential as a new tumor marker for colorectal cancers. Less