TO ELUCIDATE THE MECHANISM OF REMOTE ORGAN DYSFUNCTION AFTER INTESTINAL ISCHEMIA-REPERFUSION

1.磁共振波谱(MRS)技术为了解组织代谢变化提供了有用的信息。我们用这项技术研究了肠缺血再灌注(IR)对脑的影响。[活体31P MRS]肠缺血后(肠系膜上动脉阻断45min)所有大鼠存活超过7天。IR组在第2天表现为磷酸单酯(PME)和磷酸二酯(PDE)信号均显著增强，PME信号代表磷胆碱、磷乙醇胺等，PDE信号代表膜变性。与正常对照组动物相比，两组动物的pH值均降低。手术和反复麻醉可能会产生乳酸等酸性代谢物。[体外1HMRS]IR组第5天的N-乙酰天冬氨酸、肌酸、肌醇、乳酸和丙氨酸水平显著高于Sham组。这些数据提示肠道IR引起脑内代谢变化。2.Biologica…失血性休克后肠系膜淋巴对中性粒细胞的更多L活性：已有研究表明，结扎肠系膜淋巴管可预防失血性休克所致的肺损伤。我们假设失血性休克后肠系膜淋巴激活中性粒细胞(PMN)。将大鼠暴露于失血性休克(40 mm Hg，30min)后，用失血+生理盐水复苏2小时以上(HS组)。Asham组采用相同的手术方式，无出血和复苏。我们在复苏期间收集肠系膜淋巴1-2小时。超氧化物歧化酶抑制细胞色素C还原法测定超氧阴离子生成量。51Cr标记的中性粒细胞与纤维蛋白原结合活性测定PMS的黏附力，流式细胞仪检测CD11b的表达。HS组肠系膜淋巴组织超氧化物歧化酶生成、PMN黏附和CDT lb表面表达较假手术组显著增加。肠系膜淋巴可能在多器官功能障碍综合征的发病机制中起重要作用。较少

1.Magnetic resonance spectroscopy (MRS) technique provides useful information for the understanding of tissue metabolic changes. We used this technique to investigate the effect of gut ischemia-reperfusion (IR) on the brain. [In vivo 31P MRS] All rats survived more than seven days after gut IR (45min of superior mesenteric artery occlusion). The IR group showed a significant elevation of both phosphomonoesters (PME) and phosphodiesters (PDE) signals on day 2. The PME signal represents phosphocholine, phosphoethanolamine, etc., and the PDE signal indicate membrane degeneration. Compared to normal control animals, lower pH values were observed in both groups. Operation and repeated anesthesia may have produced acidic metabolites such as lactate. [In vitro 1H MRS] The IR group indicated significantly higer levels of N-acetylaspartate, creatines, myo-inositol, lactate and alanine than the sham group on the day 5. These data suggest gut IR provokes metabolic changes in the brain.2.Biologica … More l activities of mesenteric lymph after hemorrhagic shock on neutrophils : Previous studies have demonstrated that the ligation of mesenteric lymph duct prevents hernorrhagic shock-induced lung injury. We hypothesized that mesenteric lymph following hemorrhagic shock activates neutrophils (PMNs). Rats were exposed to hemorrhagic shock (40mmHg, 30min) and resuscitated over 2 hours with shed blood + normal saline (shed blood x 2) (HSgroup). Asham group underwent identical procedures without hemorrhage and resuscitation. We collected mesenteric lymph between 1 and 2 hours during the resuscitation phase. Superoxide anion production was measured with the superoxide dismutase inhibitable cytochrome C reduction method. PMS adhesion was measured by the radioactivity of adherent PMNs labeled with 51Cr to fibrinogen, and CD11b surface expression was determined by flow cytometry. Mesenteric lymph in the HS group significantly increased superoxide production, PMN adherence and CDT lb surface expression compared to the sham group. Mesenteric lymph may play a crucial role in the pathogenesis of multiple organ dysfunction syndrome. Less