Development ofa novel therapeutic drug for treatment of uterine cervical incompetence

这次我们继续研究MU对人宫颈成纤维细胞透明质酸合成的影响。研究在细胞因子存在的情况下，MU是否会抑制细胞内HA的合成，而HA在子宫颈成熟和分娩中起着重要的作用。方法对手术时获得的人宫颈成纤维细胞进行培养。在培养液中加入0.1 ~ 50ng/ml的IL-1 β、IL-6和IL-8。将培养细胞分成添加MU组和未添加MU组，培养48小时后收集培养基。以HA结合蛋白为原料，采用三明治结合蛋白法测定HA的含量。结果IL-8和IL-6添加组人子宫颈成纤维细胞合成HA的数量明显高于不添加细胞因子的对照组。而MU对HA的抑制作用均在50%以下。结论MU几乎完全抑制了在子宫颈成熟中起重要作用的透明质酸的产生，提示MU对子宫颈成熟具有抑制作用。因此，我们认为MU可能是一种治疗宫颈功能不全和预防早产的新型治疗药物。

We continued study about the effect of MU for hyaluronic acid synthesis in human cervical fibroblast this time. It was studied whether HA synthesis in the cell was inhibited under cytokine presence, which play an important role for cervical ripening and onset og labor, by MU addition.METHODSHuman cervical fibroblasts obtained at the time of an operation was cultured.It was added IL-1 β,IL-6 and IL-8(0.1〜50ng/ml) to a cultured medium.The medium was collected after it was divided a cultured cell more into a group of MU addition, a group of non-addition, and it was cultured for 48 hours. The amount of HA was measured by sandwitch binding protein assay method with HA binding protein.REAULTSThe amount of HA synthesized by human cervix fibroblasts increased in IL-8, an IL-6 addition group in comparison with a group of contorol(a cytokine additive-free group) significantly. However, the amount of HA was inhibited in less than 50% in all groups by addition of MU.CONCLUSIONThe finding that MU almost completely inhibited production of hyaluronic acid, which plays an important role in ripening of the cervix, suggested an inhibitory effect of MU on ripening of the cervix. Therefore, we propose that MU may be a novel therapeutic drug to treat cervical incompetence, and to prevent preterm delivery.