Cloning of differentially expressed genes in highly and low metastatic human ovarian cancer by microarray

转移包括在靶器官的侵袭、转移和增殖等多个步骤。转移相关分子，如细胞外基质蛋白酶及其抑制剂已经被发现了一段时间，但我们对转移的复杂机制仍然知之甚少。不同标本的差异表达基因可以通过基因芯片并行分析来检测。该技术具有许多优点，其中最大的优点是改进了传统的实验，允许在一次测试中观察单个或多个基因表达差异。越来越多的cDNA微阵列技术被应用于基因表达的研究。本文采用基因芯片技术，分析了高转移性人卵巢癌细胞系ykt-m及其母细胞系ykt和低转移性人卵巢癌细胞系中不同基因的表达模式。与ykt相比，共发现EGR1和胎盘钙结合蛋白（calvasculin） 2个基因表达量明显增大，转移潜力低。共发现维甲酸受体β、brca1相关环结构域蛋白、整合素β 8前体、成纤维细胞生长因子、rbq1视网膜母细胞瘤结合蛋白、hla - drantigen相关不变亚基、MHCⅱ类hla - dr - β前体、裂解刺激因子77-kDa亚基8个基因表达水平显著降低。cDNA微阵列技术可有效筛选两种人卵巢癌细胞系（ykt-m; ykt）之间的差异基因表达。这些基因可能与卵巢癌的发生和转移有关。利用基因芯片分析人卵巢癌基因表达谱，有助于基因诊断、治疗和预防。

Metastatic consists of multiple steps including invasion, transportation and proliferation in target organs.Metastasis associated molecules, like extracellular matrix proteinases and their inhibitors have been identified for some time, but we are still far from understanding the complex mechanisms operating in metastasis.Differentially expressed genes from different specimens may be detected with parallel analysis by gene chips. This technique possesses many advantages, of which the greatest is to improve on traditional experiments by allowing a single or several gene expression differences to be observed in a single test. More cDNA microarray methods are being applied in the study of gene expression. In this paper, a gene chip technique was used to analyse the different gene expression patterns in the highly potential metastatic human ovarian cancer cell line, ykt-m, and its mother cell line, ykt, as well as in low potential metastatic human ovarian cancer cell line.A total of 2 genes, which were EGR1 and placental calcium-binding protein(calvasculin), with expression levels significantly larger were found by comparing the ykt, which had low potential metastasis. A total of 8 genes, which were retinoic acid receptor beta, BRCA1-associated ring domain protein, integrin beta8precursor, fibroblast growth factor, RBQ1retinoblastoma binding protein, HLA-DRantigen-associated invariant subunit, MHC class II HLA-DR-beta precursor, and cleavage stimulation factor 77-kDa subunit, with expression levels were significantly lower were found.cDNA microarray techniques are effective in screening differential gene express ion between two human ovarian cancer cell lines (ykt-m ; ykt).These genes may be related to the genesis and metastasis of ovarian carcinoma.Analysis of the human ovarian cancer gene express ion profile with cDNA microarray may help in gene diagnosis, treatment and prevention.