Maternal Fatal cell traffic by DNA analysis and New approach for Autoinunune disorder

我们以前曾报道，用T依赖抗原免疫怀孕小鼠，成功地诱导了子代对相关抗原的抗原特异性空斑形成细胞(PFC)反应的抑制。这种抑制不是由注射的抗原、孕妇产生的抗体或哺乳期转移引起的，而是依赖于完整的母体T细胞的存在。这是一种主要组织相容性复合体(MHC)限制性的耐受方式，这种耐受至少持续了六分之一的海洋生物。传统上，胎盘扮演着天然屏障的角色，不让细胞通过。然而，这些结果强烈地表明，母体T细胞通过胎盘，随后诱导耐受。在这项研究中，我们试图通过使用分子技术证实母体细胞在胎儿循环中的存在。我们发现在H-2复合体的II类EB基因内的高度多态的微卫星序列对于各种H-2等位基因的分子检测是有用的。用DNA多态分析方法追踪小鼠脾中母体的H-2等位基因，根据我们的基本方法，在获得满意的组织知情同意后，接下来我们试图证明母体细胞在人体系统中的运输。对11岁和13岁的母亲及其子女进行了A、B、C、DR、DQ等位点的检测。但我们还没有获得完整的母细胞运输，显示了她的孩子从母亲那里获得了全套的HLA基因座。我们要增加病例数，选择SLE等自身免疫综合征患者。

We have previously reported that the immunization of pregnant mice with T-dependent antigens successfully induced suppression of the antigen-specific plaque-forming cell (PFC) response to the relevant antigens in the offspring. This suppression was not caused by the administered antigens, the antibodies produced by the pregnant mother or lactational transfer, but was dependent on the presence of the intact maternal T cells. It was major histocompatibility complex (MHC)-restricted manner tolerance, which continued for at least one-sixth of the marine life. Traditionally, the placenta acts as a natural barrier, not allowing the cells to pass through. However, the results presented strongly suggested that maternal T cells pass through the placenta and subsequently induce tolerance. In this present study, we attempted to substantiate the presence of maternal cells in the fetal circulation through the use of molecular techniques. We found that a highly polymorphic microsatellite sequence within the class II Eb gene of the H-2 complex is useful for the molecular detection of various H-2 alleles. DNA polymorphic analysis was used for tracking maternal H-2 alleles in the spleens of baby mice.According to our fundamental approaches, next we tried to prove the maternal cell trafficking in human system, after satisfactory organizing informed consents. We tried to picked up the HLA locus A, B, C, DR, DQ of mother and her children, aged 11 and 13 years old. But we have not yet obtained complete trafficking of mother cell that showed whole set of HLA locus from mother in her children. We have to increase the case and select the patient of autoimmune syndrome such as SLE etc.